Mono-Methyl-Histone H3(K9) Monoclonal Antibody

Overview of Mono-Methyl-Histone H3(K9) Monoclonal Antibody

Mono-Methyl-Histone H3(K9) Monoclonal Antibody is a laboratory reagent designed to specifically detect the monomethylation of lysine residue 9 (K9) on histone H3. Histones are core proteins around which DNA wraps to form nucleosomes, and post-translational modifications like methylation play critical roles in regulating chromatin structure, gene expression, and cellular processes .

Key Notes:

• Specificity: RevMab’s RM150 antibody shows no reactivity with non-methylated K9, H3K9me2, H3K9me3, or other histone methylations .

• Conjugation: Most products are unconjugated, suitable for secondary antibody pairing .

Epigenetic Role of H3K9me1

• Gene Regulation: H3K9me1 is associated with active enhancers and gene promoters, facilitating transcriptional activation .

• Disease Relevance: Aberrant H3K9 methylation is linked to cancer and neurological disorders, making this antibody valuable for studying epigenetic dysregulation .

Experimental Validation

• ChIP Data: RevMab’s RM150 antibody enriched DNA regions near active genes (e.g., MYC) in HeLa cells .

• WB and ICC: Consistent detection of H3K9me1 in HeLa cells, with nuclear localization confirmed via DAPI counterstaining .

Product Variations and Selection Criteria

Selection Tips:

• ChIP Studies: Prioritize RM150 (RevMab) or EPR16989 (Abcam) .

• IHC-P: Use high-dilution antibodies (e.g., ab176880 at 1:2000) .