MORC1 Antibody
Description
MORC1 Antibody Characteristics
The MORC1 antibody (e.g., Proteintech 14080-1-AP) is a polyclonal rabbit IgG antibody developed against a MORC1 fusion protein. Key properties include:
Role in Transposon Repression
MORC1 is essential for silencing transposable elements (TEs) in the male germline. Studies in mice show that MORC1 deficiency leads to:
-
Failed DNA methylation at specific TE loci, particularly LINE1 and IAP retrotransposons ( ).
-
Ectopic expression of TEs during embryonic development (E16.5–P14.5), disrupting spermatogenesis ( ).
-
Loss of H3K9me3 heterochromatin marks on activated TEs, impairing chromatin compaction ( ).
Chromatin Remodeling
-
In C. elegans, MORC-1 facilitates nuclear RNA interference (RNAi) and transgenerational epigenetic inheritance by topologically entrapping DNA and forming loops ( ).
-
In Arabidopsis, MORC1/6 homologs compact pericentromeric heterochromatin ( ).
Neurological and Mood Regulation
-
MORC1 is implicated in mood regulation, with studies linking its dysregulation to early-life stress and depression in rodents ( ).
Key Studies Using MORC1 Antibody
Technical Challenges
-
Specificity Issues: Polyclonal MORC1 antibodies often produce non-specific bands in Western blots, particularly in brain lysates, due to cross-reactivity with unknown proteins ( ).
-
Sensitivity: High protein loads (e.g., 100 µg of brain lysate) are required for detection, increasing background noise ( ).
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- These results provide evidence that MORC1 is associated with transposable elements (TEs) and suggest that a subset of these TEs may contribute to the regulation of their proximal defense genes. PMID: 27482822
- Mutations in AtMORC1 and AtMORC6 lead to derepression of DNA-methylated genes and transposable elements without affecting DNA or histone methylation. AtMORC1 and AtMORC6 belong to the conserved Microrchidia (MORC) ATPase family, predicted to catalyze changes in chromosome structure. [MORC1] PMID: 22555433
- MORC1 plays a role in pathogen-associated molecular pattern (PAMP)-triggered immunity, basal resistance, non-host resistance, and systemic acquired resistance. PMID: 23250427
- CRT1, an ATPase, is a crucial mediator of defense signaling triggered by different classes of R proteins. [CRT1] PMID: 18191794
