MORC1 Antibody, FITC conjugated

Target Protein Overview

MORC1 (UniProt ID: Q86VD1) is a 112.9 kDa nuclear protein critical for:

• Chromatin compaction and heterochromatin formation

• Transposable element (TE) silencing during spermatogenesis

• DNA methylation processes in germ cells

Key functional domains include an N-terminal GHKL ATPase domain and C-terminal CW-type zinc finger motifs .

Chromatin Dynamics Studies

• Compacts DNA through loop formation (≤50% size reduction in vitro)

• Restores H3K9me3 heterochromatin marks on piRNA-targeted transposons

Germ Cell Development

• Essential for silencing 9,152+ TEs in mouse gonocytes

• Interacts with SetDB1 methyltransferase for heterochromatin reestablishment

Technical Performance

• Detects MORC1 at 108 kDa in WB under reducing conditions

• Stable in PBS with 50% glycerol at -20°C for ≥12 months

DNA Compaction Efficiency

Data from single-molecule imaging shows concentration-dependent chromatin remodeling .

TE Silencing Impact

Knockout models demonstrate complete loss of heterochromatin stability on Group I TEs .

Quality Control Metrics

• Purity: >70% by SDS-PAGE and size-exclusion chromatography

• Specificity: Validated in FcRγc KO models to exclude cross-reactivity

• Lot Consistency: ≤15% variance in ELISA titers across production batches

Limitations and Considerations

• Requires antigen retrieval in formalin-fixed paraffin-embedded tissues

• Potential background in IgG-rich samples due to Fc domain interactions

• Not validated for flow cytometry in hematopoietic cells