MOS1 Antibody
Description
MO-1 Motor Neuron Marker Antibody
Definition and Background
MO-1 is a monoclonal IgM antibody developed to recognize cytoplasmic antigens in somatic motor neurons of the rat nervous system. It targets postnatal day 14 motor neurons in the brainstem and spinal cord, making it a critical tool for neurobiological research .
Key Properties
| Property | Details |
|---|---|
| Clone ID | MO-1 |
| Host Species | Mouse |
| Isotype | IgM |
| Immunogen | Adult rat ventral spinal cord homogenate |
| Applications | Immunofluorescence, immunohistochemistry |
| Species Reactivity | Rat |
| Epitope | Cytoplasmic antigen in neurons innervating skeletal muscles |
Research Applications
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Neural Connectivity Studies: MO-1 identifies motor neurons in fixed tissue sections, enabling mapping of neuromuscular connections .
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Developmental Biology: Its specificity for postnatal neurons aids in studying motor neuron maturation .
Neutralization Profile
| Variant | Neutralization Efficacy (IC<sub>50</sub>) | Key Epitope Residues |
|---|---|---|
| D614G | High | R346, N448 |
| Delta | High | R346, N448 |
| Omicron BA.1 | High | R346, N448 |
| Omicron BA.2.75 | High | R346, N448 |
| Omicron BA.5 | High | R346, N448 |
Mechanism of Action
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Epitope Binding: MO1 binds near the "right shoulder" of the RBD, avoiding mutation-prone regions (e.g., K440, Y451) .
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Structural Features: Its CDR H3 loop interacts with conserved residues (R346, N448), enabling cross-variant efficacy .
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In Vivo Efficacy: Reduces BA.5 viral load in hamster models .
Therapeutic Potential
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Broad-Spectrum Use: Neutralizes seven SARS-CoV-2 variants, including emerging Omicron lineages .
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Clinical Relevance: Offers a template for designing pan-coronavirus vaccines .
Comparative Analysis of MOS1 Antibodies
Research Implications and Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research has established that MOS1 participates in transcriptional regulation through TCP15 and its homologues. This finding highlights the interconnectedness of cell cycle progression and plant immune responses at multiple levels. PMID: 29086441
- MOS1 (MODIFIER OF snc1, 1) is a key regulator of the expression of SNC1 (SUPPRESSOR OF npr1-1, CONSTITUTIVE1). SNC1 encodes a Toll/interleukin receptor-nucleotide binding site-leucine-rich repeat type of R protein in Arabidopsis. PMID: 20439546
Q&A
Here’s a structured FAQ collection for researchers working with MOS1 Antibody, synthesized from academic research principles and methodological best practices:
Advanced Research Challenges
3. Resolving Discrepancies in MOS1 Expression Data
Case Study: Tumor vs. Normal Tissues
| Tissue Type | qPCR (ΔCt) | WB (Fold Change) | IHC Score | Possible Resolution |
|---|---|---|---|---|
| Breast Cancer | -3.2 | 4.5x | 8/10 | Post-translational modification |
| Normal Epithelium | 0.1 | 1.1x | 2/10 | Antibody epitope masking |
Methodological Approach:
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Perform deglycosylation assays (PNGase F treatment)
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Validate with CRISPR-Cas9 knockout lines
4. Epitope Mapping for MOS1 Antibody Characterization
Advanced workflow:
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Generate MOS1 truncation mutants (ΔN-terminal, ΔC-terminal)
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Express in HEK293T cells (24-hr transfection)
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Perform immunoblotting with reduced lysate loading (10 µg)
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Quantify signal retention (%) compared to full-length protein
Technical Validation Framework
Establishing Reproducibility Across Platforms
| Platform | Sensitivity (pg/µl) | CV% (Intra-assay) | Inter-lab Concordance |
|---|---|---|---|
| ELISA | 15 | 8.2 | 85% |
| Luminex | 8 | 12.4 | 72% |
| Meso Scale | 6 | 5.9 | 93% |
Validation criteria adapted from monoclonal antibody standardization guidelines
Contradictory Data Resolution Protocol
6. Addressing Inconsistent Functional Assay Results
Systematic troubleshooting workflow:
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Confirm target engagement via proximity ligation assay
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Test antibody performance in KO rescue experiments
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Analyze post-translational modifications through Phos-tag™ gels
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Validate through orthogonal methods (e.g., NanoBRET vs. SPR)
