MPK3 Antibody
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Constitutive active-MPK3 plants exhibit enhanced resistance to the hemibiotrophic pathogen Pseudomonas syringae DC3000. PMID: 28766995
- Constitutively active (CA)-MPK3, when crossed with summ1 and summ2, known suppressors of mpk4, partially reverted the CA-MPK3 phenotypes. PMID: 28400495
- Alterations in PUB22 ubiquitination modes induced by MITOGEN-ACTIVATED PROTEIN KINASE3 dampen the immune response. PMID: 28280093
- MPK3 is implicated in stomatal closure induced by ultraviolet radiation. PMID: 27837091
- The MKK3-MPK6 module is activated by blue light in a MYC2-dependent manner. PMID: 25139007
- MicroRNA biogenesis factor DRB1 is a phosphorylation target of mitogen activated protein kinase MPK3 in both rice and Arabidopsis. PMID: 25417716
- MPK3 represses the constitutive and flg22-induced expression of defense genes in Arabidopsis thaliana. PMID: 24980080
- AZI1 overexpression in the mpk3 genetic background partially alleviates the salt-hypersensitive phenotype of this mutant, suggesting that functional MPK3 is required for the full extent of AZI1-conferred robustness. PMID: 24214892
- MYB44 is regulated by MPK3 via phosphorylation, initiating the adaptation response to various abiotic and biotic stresses. PMID: 23437396
- Amino acids surrounding the phosphorylated serine residue of peptide substrates contribute to MPK3/MPK6 substrate specificity, highlighting the importance of MPK3/MPK6 activities in stomatal growth. PMID: 22631074
- MPK3 plays a role in basal and oligogalacturonide- or flagellin-induced resistance against Botrytis cinerea. PMID: 21803860
- MKK4 is involved in the osmotic-stress response through its regulation of MPK3 activity. PMID: 21806969
- Studies have shed light on the molecular mechanisms controlling AtMPK3 expression in response to different environmental stimuli. PMID: 21046323
- MPK3 phosphorylates ACS2 and ACS6, and MPK3 and MPK6 are essential for ethylene induction in Arabidopsis seedlings infected by B. cinerea. PMID: 20659280
- Ozone (O3) treatment, which generates reactive oxygen species in the apoplast of Arabidopsis thaliana, causes a transient activation of mitogen-activated protein kinase AtMPK3. PMID: 15500467
- MPK3 is a key regulator of stomatal development and patterning. PMID: 17259259
- Research demonstrates a significant role of MPK3 in the response to abscisic acid and hydrogen peroxide in guard cells. PMID: 17286820
- VIP1 is a direct target of Agrobacterium-induced MPK3. Upon phosphorylation by MPK3, VIP1 translocates to the nucleus and regulates the expression of PR1. MAPK-dependent phosphorylation of VIP1 is crucial for VIP1-mediated Agrobacterium T-DNA transfer. PMID: 17947581
- MPK3 plays an essential role in promoting cell division in the integument specifically during ovule development. PMID: 18364464
- The MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection. PMID: 18378893
- VIP1 binding to VIP1 response elements is enhanced under conditions of MPK3 pathway stimulation. PMID: 19820165
Q&A
Basic Research Questions
How to validate MPK3 antibody specificity in Arabidopsis thaliana experiments?
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Perform immunoblotting using mpk3 knockout mutants (e.g., mpk3-1 or MPK3-DG lines) as negative controls .
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Use peptide competition assays with MPK3-specific epitopes to confirm binding specificity .
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Compare signal intensity across wild-type, mpk3 mutants, and transgenic lines overexpressing MPK3 homologs (e.g., MPK6) to rule out cross-reactivity .
What controls are essential for MPK3 detection in Western blot assays?
Advanced Research Questions
How to resolve conflicting data on MPK3 activation kinetics under stress conditions?
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Standardize elicitor treatments (e.g., oligogalacturonides or flg22) across experiments to minimize variability .
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Combine phospho-specific MPK3 antibodies with kinase activity assays (e.g., immunoprecipitation followed by in vitro kinase assays) .
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Validate temporal phosphorylation patterns using time-course experiments and parallel monitoring of MPK6 activation to differentiate signaling roles .
What methodological approaches distinguish MPK3 from MPK6 in dual MAPK activation studies?
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Use isoform-specific antibodies validated against mpk3 and mpk6 mutants .
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Implement RNAi lines (e.g., MPK6-RNAi) to selectively silence one kinase while monitoring the other .
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Analyze phosphorylation dynamics via targeted mass spectrometry to identify unique peptide signatures .
How to address MPK3 antibody cross-reactivity in non-model plant species?
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Perform sequence alignment of MPK3 epitopes across target species to predict antibody compatibility .
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Validate using heterologous expression systems (e.g., transient expression of MPK3 in Nicotiana benthamiana followed by immunoblotting) .
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Combine with CRISPR-generated mutants in the target species to confirm antibody specificity .
Data Contradiction Analysis
How to interpret discrepancies in MPK3 subcellular localization studies?
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Assess antibody validation protocols: Poorly validated antibodies may produce artifactual localization patterns .
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Compare fixation methods (e.g., formaldehyde vs. methanol) to rule out fixation-dependent epitope masking .
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Use complementary techniques like GFP-tagged MPK3 lines for live-cell imaging .
Why do some studies report MPK3 involvement in salicylic acid (SA) signaling while others do not?
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Evaluate genetic backgrounds: mpk3 mutants with SA hyperaccumulation (e.g., mpk4 double mutants) may exhibit indirect effects .
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Distinguish between basal resistance (MPK3-dependent) and induced resistance (MPK6-dominated) pathways .
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Use transcriptional profiling to isolate MPK3-specific SA-responsive genes (e.g., ALD1 or PBS3) .
