MRPL10 Antibody
Description
MRPL10 Protein and Its Role in Mitochondria
MRPL10 is encoded by the MRPL10 gene (OMIM: 611825) and localizes to the mitochondrial large ribosomal subunit (39S). It plays a central role in mitochondrial protein synthesis, which is essential for producing components of the electron transport chain and ATP synthase . Unlike bacterial ribosomes, mitochondrial ribosomes in mammals have a higher protein-to-rRNA ratio (~75%) and lack 5S rRNA, making them structurally distinct .
Key Features of MRPL10:
-
Post-Translational Modification: MRPL10 undergoes lysine acetylation, which regulates mitochondrial translation efficiency. SIRT3, an NAD⁺-dependent deacetylase, deacetylates MRPL10 to modulate ribosomal activity .
-
Sequence Variability: MRPL10 exhibits low sequence homology with bacterial homologs, complicating evolutionary analyses .
Regulatory Role in Mitochondrial Translation
In a landmark study, SIRT3 was identified as the primary deacetylase for MRPL10. Acetylation at lysine residues (K124, K162, K196) enhances ribosomal activity, while deacetylation by SIRT3 suppresses it . This mechanism links mitochondrial translation to cellular energy status via NAD⁺ levels.
Antibody-Validated Experimental Models
-
Western Blot (WB):
-
Immunoprecipitation (IP):
-
Immunofluorescence (IF):
Best Practices for Experimental Use
Product Specs
Target Background
- This antibody is designed to analyze the RNA binding properties of orthologous bovine 39S ribosomal subunit proteins, including MRPL10. PMID: 3129699
Q&A
Basic Research Questions
-
What is MRPL10 and what is its role in mitochondrial function?
MRPL10 (mitochondrial ribosomal protein L10) is a component of the large subunit (39S) of the mitochondrial ribosome. It plays a crucial role in mitochondrial protein synthesis. Research has identified MRPL10 as the major acetylated protein in the mitochondrial ribosome, suggesting it serves as a regulatory node for mitochondrial translation . The protein has a molecular weight of 29 kDa and consists of 261 amino acids .
Methodologically, researchers studying MRPL10 function often employ techniques like RNA interference (RNAi) to deplete MRPL10 and observe effects on mitochondrial translation rates and mitochondrial function. Similar to findings with MRPL12, MRPL10 depletion may affect steady-state levels of mitochondrial transcripts .
-
What applications are MRPL10 antibodies commonly used for in research?
MRPL10 antibodies are versatile tools in mitochondrial research, with validated applications including:
Application Typical Dilution Range Cell/Tissue Types Western Blot (WB) 1:500-1:50000 HEK-293, HeLa, A549, U2OS, LNCaP, Jurkat, K-562 cells Immunohistochemistry (IHC) 1:20-1:200 Human testis tissue Immunofluorescence (IF)/ICC 1:50-1:1600 HepG2, HeLa cells Immunoprecipitation (IP) 0.5-4.0 μg for 1.0-3.0 mg protein Mouse liver tissue ELISA 1:1000 Various Cytometric bead array Range: 0.098-100 ng/mL Various For optimal results, researchers should perform antibody titration in each experimental system .
-
What species reactivity do commercially available MRPL10 antibodies demonstrate?
MRPL10 antibodies show varying species reactivity profiles depending on the manufacturer and clone. Based on validation data:
Antibody Identifier Host Species Reactivity Validation Method 16652-1-AP (Proteintech) Rabbit Human, mouse, rat WB, IHC, IF/ICC, IP 68623-1-Ig (Proteintech) Mouse Human WB, IF/ICC ABIN3185640 Rabbit Human WB, ELISA, IHC HPA021234 Rabbit Human WB, IF, IHC NBP1-82858 Rabbit Human WB, IHC, IF When selecting an antibody for cross-species studies, researchers should prioritize those with validated reactivity across their species of interest .
-
How should researchers properly store and handle MRPL10 antibodies?
Proper storage and handling of MRPL10 antibodies is critical for maintaining their performance and extending shelf life. Based on manufacturer recommendations:
-
Storage temperature: Store at -20°C for long-term preservation
-
Buffer composition: Most MRPL10 antibodies are provided in PBS with 0.02% sodium azide and 50% glycerol at pH 7.3
-
Aliquoting: While manufacturers indicate aliquoting is unnecessary for -20°C storage, it is recommended for antibodies that will undergo multiple freeze-thaw cycles
-
Stability: MRPL10 antibodies are typically stable for one year after shipment when stored properly
-
Working solution: When preparing diluted working solutions, use fresh buffer and store at 4°C for short-term use only
Some suppliers offer smaller (20μl) sizes containing 0.1% BSA for enhanced stability . Always consult the product-specific documentation for any special handling considerations.
-
-
What controls should be included when working with MRPL10 antibodies?
Proper experimental controls are essential for validating MRPL10 antibody results:
Positive controls:
-
Cell lines with known MRPL10 expression: HEK-293, HeLa, A549, and HepG2 cells
-
Mitochondrial fraction: Enriched mitochondrial preparations from HEK-293 cells
Negative controls:
-
Primary antibody omission: Replacing primary antibody with same-species IgG
-
MRPL10 knockdown/knockout: Using siRNA or CRISPR to generate MRPL10-depleted samples
-
Blocking peptide: Pre-incubation of antibody with immunizing peptide to confirm specificity
Specificity validation:
-
Western blot should show a single band at the expected molecular weight (29 kDa)
-
Immunofluorescence should demonstrate mitochondrial localization pattern
-
Knockdown validation: Signal should decrease proportionally to protein depletion
Methodologically, researchers should include these controls in every experiment to ensure reliable interpretation of results.
-
