MRPL1 Human

Mitochondrial Ribosomal Protein L1 Human Recombinant
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Cat. No.
BT13352
Source
E.coli.
Synonyms
BM022, L1MT, MRP-L1, 39S ribosomal protein L1, mitochondria, MRPL1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Introduction to MRPL1 Human

MRPL1 (mitochondrial ribosomal protein L1) is a nuclear-encoded protein critical for mitochondrial translation in humans. Encoded by the MRPL1 gene located on chromosome 4q21.1 , it is a component of the mitochondrial large ribosomal subunit (39S) and plays a central role in synthesizing mitochondrial proteins essential for cellular energy production.

Gene Structure and Chromosomal Localization

AttributeDetail
Gene SymbolMRPL1
Chromosomal Location4q21.1
Exons8 exons spanning ~15 kb
TranscriptsNM_020236.3 (primary) ; alternative splicing produces isoforms
Protein Length325 amino acids (isoform 1)
Molecular Weight~33.8 kDa (recombinant form)

Functional Role in Mitochondrial Translation

MRPL1 belongs to the L1 ribosomal protein family and contributes to the structural integrity of the 39S subunit. Key functions include:

  • Translation Elongation: Facilitates peptide bond formation during mitochondrial mRNA translation .

  • Ribosomal Stability: Maintains the 39S subunit’s conformation, enabling interactions with mitochondrial mRNA and transfer RNA (tRNA) .

  • Protein Synthesis Regulation: Collaborates with other mitochondrial ribosomal proteins (MRPs) to coordinate translation machinery .

Breast Cancer Prognosis

MRPL1 is upregulated in breast cancer (BC) and correlates with poor survival outcomes . Key findings from a 2022 study include:

Mechanistic Insight: MRPL1 overexpression disrupts mitochondrial translation, potentially driving cancer progression via metabolic reprogramming .

Protein Structure and Biochemical Properties

PropertyDetail
Amino Acid Sequence325 residues (isoform 1); contains conserved motifs for ribosomal binding
Post-Translational ModsPhosphorylation (predicted)
Subcellular LocalizationMitochondria (maternal lineage)
Recombinant ProductionExpressed in E. coli with N-terminal His-tag (33.8 kDa)

Interaction Network and Functional Partners

MRPL1 interacts with other mitochondrial ribosomal proteins and translation factors:

Interaction PartnerFunction
MRPL4Stabilizes ribosomal structure
MRPL11Binds rRNA and facilitates translation initiation
MRPL32Involved in ribosome assembly
DAP3Regulates mitochondrial translation termination

Applications in Research and Diagnostics

ApplicationDetails
AntibodiesMonoclonal (e.g., clone 2B7) and polyclonal antibodies for immunoblotting
Protein StudiesRecombinant MRPL1 used for structural analysis and interaction assays
Cancer BiomarkersIncluded in MRP-based prognostic nomograms for breast cancer

Expression Profile Across Tissues and Cell Lines

MRPL1 exhibits ubiquitous expression due to its essential role in mitochondrial protein synthesis:

Tissue/Cell LineExpression Level
LiverHigh (central role in energy metabolism)
HeartHigh (isoform 2 predominant)
Breast Cancer CellsElevated (SUM102PT exception)
SUM102PT (BC Cell Line)Lowest expression observed

Product Specs

Introduction
Mitochondrial Ribosomal Protein L1 (MRPL1) is a crucial component of the large (39S) subunit of mitochondrial ribosomes, which are essential for protein synthesis within mitochondria. These ribosomes, distinct from cytoplasmic ribosomes, are composed of a 39S large subunit and a 28S small subunit. MRPL1, alongside other mitochondrial ribosomal proteins (MRPs), plays a vital role in this process. The MRPL1 gene undergoes alternative splicing, resulting in two isoforms. Isoform 1 is expressed ubiquitously, while isoform 2 shows preferential expression in the heart.
Description
Recombinant human MRPL1, expressed in E. coli, is available as a purified single polypeptide chain. This protein encompasses amino acids 51 to 325 of the MRPL1 sequence, resulting in a molecular weight of 33.8 kDa. For purification and detection purposes, a 23-amino acid His-tag is fused to the N-terminus. The protein is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The MRPL1 solution is provided at a concentration of 0.5 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 30% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the MRPL1 protein is greater than 80%, as assessed by SDS-PAGE analysis.
Synonyms
BM022, L1MT, MRP-L1, 39S ribosomal protein L1, mitochondria, MRPL1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSKKTKKGA KEKTPDEKKD EIEKIKAYPY MEGEPEDDVY LKRLYPRQIY EVEKAVHLLK KFQILDFTSP KQSVYLDLTL DMALGKKKNV EPFTSVLSLP YPFASEINKV AVFTENASEV KIAEENGAAF AGGTSLIQKI WDDEIVADFY VAVPEIMPEL NRLRKKLNKK YPKLSRNSIG RDIPKMLELF KNGHEIKVDE ERENFLQTKI ATLDMSSDQI AANLQAVINE VCRHRPLNLG PFVVRAFLRS STSEGLLLKI DPLLPKEVKN EESEKEDA.

Product Science Overview

Introduction

Mitochondrial Ribosomal Protein L1 (MRPL1) is a crucial component of the mitochondrial ribosome, specifically the large 39S subunit. This protein is encoded by the MRPL1 gene in humans and plays a significant role in mitochondrial protein synthesis .

Gene and Protein Structure

The MRPL1 gene is located on chromosome 4q21.1 in humans . It encodes a protein that is part of the L1 ribosomal protein family. The mitochondrial ribosome, or mitoribosome, consists of a small 28S subunit and a large 39S subunit. Unlike prokaryotic ribosomes, mitoribosomes have a higher protein to rRNA ratio (approximately 75% protein) and lack a 5S rRNA .

Function

MRPL1 is involved in the mitochondrial translation process, which is essential for the synthesis of proteins within the mitochondria. These proteins are critical for the mitochondrion’s role in energy production and other metabolic processes . The protein’s functions include:

  • RNA binding: MRPL1 binds to RNA, which is crucial for its role in the ribosome.
  • Structural constituent of ribosome: It helps maintain the structure and function of the ribosome .
Pathways

MRPL1 is associated with several biological pathways, including:

  • Mitochondrial translation: The process by which proteins are synthesized within the mitochondria.
  • Metabolism of proteins: Involves the synthesis, folding, and degradation of proteins .
Clinical Significance

Mutations or dysfunctions in the MRPL1 gene can potentially lead to mitochondrial diseases, which are often characterized by defects in energy production. These conditions can affect various organs and systems, leading to a wide range of clinical manifestations .

Research and Applications

Recombinant MRPL1 protein is used in research to study mitochondrial function and diseases. It is also utilized in the development of therapeutic approaches for mitochondrial disorders .

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