MRPS35 Antibody
Description
Applications in Research
The antibody is validated for multiple techniques:
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Western Blotting (WB): Detects MRPS35 in mitochondrial extracts, with recommended dilutions of 1:500–1:2000 .
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Immunohistochemistry (IHC): Stains mitochondrial structures in tissues (e.g., A549, HT-29 cells) .
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Immunofluorescence (IF): Visualizes mitochondrial localization in live/dead cells .
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ELISA: Used in sandwich assays for quantifying MRPS35 in biological fluids .
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Immunoprecipitation (IP): Purifies MRPS35-protein complexes .
Cancer Biology
MRPS35 antibodies have been pivotal in studying hepatocellular carcinoma (HCC). A circular RNA isoform, circMRPS35, derived from MRPS35, promotes HCC progression by sponging miR-148a-3p and stabilizing PTEN degradation pathways . Additionally, circMRPS35 encodes a 168-amino acid peptide that confers cisplatin resistance in HCC cells .
Virology
In arenavirus studies, MRPS35 antibodies co-localized viral nucleoproteins (NP) within mitochondrial matrices, suggesting mitochondrial hijacking mechanisms .
Product Variants and Comparisons
Multiple commercial variants exist, differing in reactivity and conjugation:
