MRS2-7 Antibody
Description
MRS2 Antibodies: Overview
MRS2 (Magnesium Homeostasis Factor Homolog) is a mitochondrial inner membrane protein critical for Mg²⁺ transport and mitochondrial function. Antibodies targeting MRS2 are used in research to study its role in energy metabolism, mitochondrial diseases, and Mg²⁺ regulation.
Key MRS2 Antibodies and Their Characteristics
2.1. N-Glycosylation and Mitochondrial Mg²⁺ Flux
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N-Glycosylated MRS2 Isoforms: Antibodies (e.g., ABIN7043335) have identified two MRS2 isoforms: glycosylated (higher molecular weight) and unglycosylated (lower molecular weight). Glycosylation reduces Mg²⁺ influx capacity, linking it to cellular energy demand (glycolysis vs. oxidative phosphorylation) .
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Disease Relevance: Increased glycosylated MRS2 is observed in mitochondrial disease patient fibroblasts, suggesting a potential diagnostic biomarker .
3.1. Western Blotting
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Detection: Antibodies like ABIN7043335 and ABIN6743527 are used to assess MRS2 expression levels in mitochondrial isolates or cell lysates .
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Glycosylation Analysis: PNGase F treatment shifts MRS2 bands, confirming post-translational modifications .
3.2. Immunohistochemistry
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Tissue Localization: NBP2-34200 stains MRS2 in human skeletal muscle (myocytes) and placental trophoblasts, highlighting its role in energy-intensive tissues .
3.3. Functional Assays
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Mitochondrial Mg²⁺ Uptake: Overexpression of hMrs2 in HEK-293F cells increases Mg²⁺ influx, validated via mitochondrial assays and antibody-based detection .
Limitations and Future Directions
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- MRS2-7 represents the first identified low-affinity Mg(2+) transporter from a higher plant. Notably, the gene encoding MRS2-7 exhibits alternative splicing, resulting in the production of proteins with distinct functional properties. PMID: 19093971
- Overexpression of MRS2-7 has been demonstrated to enhance biomass accumulation in plants subjected to magnesium-limiting conditions. PMID: 19966073
