MSI4 Antibody
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research demonstrates that PTM, a PHD transcription factor implicated in chloroplast retrograde signaling, detects such a signal and mediates transcriptional repression of FLC through the recruitment of FVE (MSI4), a component of the histone deacetylase complex. PMID: 27601637
- Data suggests that these newly identified ATMSI4-associated proteins contribute to the formation of nucleoprotein complexes that determine the pleiotropic functional properties of AtMSI4/FVE/ACG1, playing a significant role in plant development. PMID: 25600937
- FVE (MSI4) is essential for the HOS1-mediated activation of FLC transcription. PMID: 24220632
- Two phospho-beta-galactosidase, LacG1 and LacG2, are the primary enzymes responsible for lactose utilization in Lb. gasseri. PMID: 22449746
- Research indicates that two Arabidopsis homologs of the human histone-binding proteins Retinoblastoma-Associated Protein 46/48 (RbAp46/48), known as MSI4 (or FVE) and MSI5, function in partial redundancy in chromatin silencing of various loci targeted by siRNAs. PMID: 22102827
- FVE (MSI4), an Arabidopsis homologue of the retinoblastoma-associated protein that regulates flowering time and cold response, binds to chromatin as a large multiprotein complex. PMID: 21710206
- These findings suggest that there may be a conserved function between OsFVE and FVE (MSI4) in the control of flowering time. PMID: 18612241
Q&A
Here’s a structured collection of FAQs tailored for academic researchers working with MSI4 antibody, synthesized from peer-reviewed methodologies and experimental data:
Advanced Research Questions
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How to resolve contradictions in MSI4’s role in abiotic stress vs. flowering regulation?
Experimental design considerations:-
Temporal analysis: Compare early (0–3 hr) vs. late (8–24 hr) stress responses using time-course RNA-seq .
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Condition-specific mutants: Combine msi4 mutants with stress-inducible promoters (e.g., cold/mannitol/salt) to isolate context-dependent roles .
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Table 1: MSI4-associated pathways under distinct stresses :
Stress Condition Regulated Genes Overlap with Flowering Pathways Cold 1,335 genes FLC, FT Salt/Mannitol 462 genes PRC2 targets
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What advanced techniques are used to study MSI4’s chromatin remodeling activity?
Integrate these methods:-
Bimolecular Fluorescence Complementation (BiFC): Visualize MSI4-DDB1 interactions in vivo (e.g., YN-MSI4 + YC-DDB1A in mustard hypocotyls) .
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CUL4 depletion assays: Treat plants with proteasome inhibitors (e.g., MG132) to test MSI4’s dependence on CUL4 for chromatin binding .
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Multi-omics correlation: Pair ChIP-seq data with RNA-seq to link MSI4 binding to transcriptional changes at stress/flowering genes .
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Methodological Best Practices
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How to optimize ChIP protocols for MSI4 in plant tissues?
Critical steps:
Data Interpretation Guidelines
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How to distinguish between direct and indirect MSI4-mediated gene repression?
Apply these criteria:
