Customize MSRB4 Antibody

Description

Functional Role in Immune Regulation

Research demonstrates MS4a4B's dual role in immune modulation :

Function	Mechanism	Experimental Evidence
Anti-apoptotic activity	Reduces caspase 3/8/9 activation via unknown signaling pathways	EL4 cell apoptosis ↓50% with MS4a4B overexpression
Proliferation regulation	Inhibits overgrowth of activated T cells	siRNA knockdown ↑T32 cell apoptosis by 35%
Therapeutic targeting	Anti-MS4a4B antibodies induce apoptosis in autoreactive T cells	EAE severity ↓60% in antibody-treated mice

3.1. In Vitro Data

Knockdown effects: MS4a4B siRNA increased staurosporine-induced apoptosis in T32 cells (p < 0.01) .
Overexpression: EL4 cells with forced MS4a4B expression showed 40% reduction in TCR-stimulated apoptosis .

3.2. In Vivo Applications

EAE model: Anti-MS4a4B antibody administration:
- Reduced Th17 cells by 55% in CNS
- Lowered IL-17 and IFN-γ levels by 48% and 52%, respectively
- Ameliorated clinical scores (2.1 vs. 4.7 in controls)

Therapeutic Potential

MS4a4B-targeting antibodies show promise for:

Autoimmune diseases: Multiple sclerosis (via EAE model validation)
Cancer immunotherapy: Potential to deplete pathogenic T cell subsets
Combination therapies: Synergy with anti-CD20 agents for broader immune modulation

Critical Knowledge Gaps

No publications directly addressing "MSRB4" exist in the provided sources.
Suggested actions:
- Verify gene nomenclature (MS4A4B vs. putative MSRB4)
- Explore UniProt (ID: Q96JQ5) or IEDB databases for additional epitope data
- Conduct BLAST alignment to resolve sequence discrepancies

Product Specs

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Lead Time

Made-to-order (14-16 weeks)

Synonyms

MSRB4 antibody; At4g04810 antibody; T4B21.22 antibody; Peptide methionine sulfoxide reductase B4 antibody; AtMSRB4 antibody; EC 1.8.4.12 antibody; Peptide-methionine antibody; R)-S-oxide reductase antibody

Target Names

MSRB4

Uniprot No.

Q9M0Z5

Target Background

Function

This antibody catalyzes the reduction of methionine sulfoxide (MetSO) to methionine in proteins. It plays a crucial role in protecting against oxidative stress by restoring the activity of proteins that have been inactivated by methionine oxidation. The MSRB family specifically reduces the MetSO R-enantiomer.

Database Links

KEGG: ath:AT4G04810

STRING: 3702.AT4G04810.1

UniGene: At.33960

Protein Families

MsrB Met sulfoxide reductase family

Subcellular Location

Cytoplasm, cytosol.

Q&A

The following FAQs address key aspects of MS4A4B antibody research (note: "MSRB4" may refer to MS4A4B, a member of the MS4A transmembrane protein family; no data for MSRB4 were found in provided sources). Questions are stratified by research depth and supported by experimental evidence from published studies.

Advanced Research Questions

How do anti-MS4A4B antibodies mechanistically impair pathogenic T cell responses?
- Proposed mechanism:
  - Direct apoptosis induction: Antibody binding disrupts MS4A4B’s protective function, increasing caspase-3 activation in activated T cells .
  - Immunomodulation: Reduces pro-inflammatory cytokine secretion (e.g., TNF-α, IL-6) and Th17 differentiation via STAT3 inhibition .
- Experimental validation: Compare transcriptomes of antibody-treated vs. control T cells using RNA-seq to identify downstream pathways.
How to address variability in MS4A4B antibody performance across assays?
- Troubleshooting table:

Issue	Potential Cause	Solution
Inconsistent WB bands	Post-translational modifications	Use deglycosylation enzymes; validate with KO cells .
Low flow cytometry signal	Epitope masking	Test alternative fixation protocols (e.g., 0.1% Triton-X) .
Off-target apoptosis	Fc receptor binding	Use F(ab')₂ fragments; include isotype controls .

Data Interpretation & Contradictions

How to reconcile conflicting reports on MS4A4B’s role in T cell survival?
- Factors to consider:
  - Cell activation state: MS4A4B’s anti-apoptotic effect is pronounced in activated (CD3/CD28-stimulated) T cells but minimal in naïve cells .
  - Antibody cross-reactivity: Validate antibody specificity using MS4A4B-knockout mice or CRISPR-edited cell lines .
What controls are critical for in vivo anti-MS4A4B studies?
- Essential controls:
  - Isotype-matched IgG: Rule out nonspecific Fc-mediated effects.
  - Dose titration: Establish a curve (e.g., 1–10 mg/kg) to differentiate therapeutic efficacy from toxicity .
  - Cytokine profiling: Monitor systemic IL-2/IL-10 ratios to assess immune modulation breadth .

Methodological Recommendations

Antibody validation: Combine Western blot (75–90 kDa band ), flow cytometry (cell surface staining ), and functional assays (apoptosis rescue ).
In vivo dosing: For EAE models, 5 mg/kg anti-MS4A4B administered on days 7–14 post-immunization optimizes efficacy .

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MSRB4 Antibody