MTMR3 Antibody, Biotin conjugated
Shipped with Ice Packs 
In Stock 
Description
Key Uses in Experimental Settings:
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ELISA: Biotin conjugation enhances detection sensitivity through streptavidin-HRP or streptavidin-fluorophore systems .
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Proximity Labeling: Facilitates spatial mapping of MTMR3 interactomes in fixed cells/tissues via antibody-guided biotin deposition .
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Autophagy Studies: MTMR3 modulates PI3P levels, influencing autophagosome formation .
Example Protocol (ELISA):
Technical Considerations
Key Research Findings
Recent studies utilizing biotinylated MTMR3 antibodies include:
Validation and Quality Control
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Western Blot: Detects bands at ~130–150 kDa in human cell lines (HeLa, HEK293) .
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Immunofluorescence: Localizes to perinuclear regions and cytoplasmic vesicles .
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Lot-Specific Data: Provided by vendors (e.g., Boster Bio’s validation images) .
Limitations and Alternatives
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Typically, we can ship products within 1-3 business days after receiving your order. Delivery times may vary depending on the purchase method or location. Please consult your local distributor for specific delivery information.
Synonyms
FYVE domain-containing dual specificity protein phosphatase 1 antibody; FYVE-DSP1 antibody; MTMR3 antibody; MTMR3_HUMAN antibody; Myotubularin-related protein 3 antibody; Zinc finger FYVE domain-containing protein 10 antibody
Target Names
Uniprot No.
Target Background
Function
MTMR3 antibody is a phosphatase that specifically targets lipids with a phosphoinositol headgroup. It exhibits phosphatase activity towards phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate. Additionally, it may dephosphorylate proteins phosphorylated on Ser, Thr, and Tyr residues.
Gene References Into Functions
- Research indicates that MTMR3 plays multiple roles in regulating autophagy. One proposed mechanism involves direct interaction between MTMR3 and mTORC1 (MTOR, mLST8, raptor), leading to the inhibition of mTORC1 phosphorylation activity. MTMR3 is localized to the Golgi apparatus. PMID: 26787466
- The rs713875 IBD risk polymorphism increases MTMR3 expression, influencing pattern recognition receptor (PRR)-induced outcomes, including enhanced caspase-1 activation. PMID: 26240347
- Myotubularin-related protein 3 (MTMR3) and myotubularin-related protein 4 (MTMR4) may serve as a bridge between CEP55 and polo-like kinase 1, ensuring proper CEP55 phosphorylation and regulating CEP55 recruitment to the midbody. PMID: 25659891
- Cloning, expression, purification, and crystallization of human MTMR3 encompassing the PH-GRAM and phosphatase catalytic domain have been reported. PMID: 25195900
- These findings suggest that MTMR3 may play a significant role in colorectal cancer (CRC) progression. siRNA-mediated silencing of MTMR3 could potentially serve as an effective therapeutic strategy in CRC treatment. PMID: 25215329
- Our research indicates that the MTMR3 gene is shared between IgA nephropathy and lupus nephritis in the northern Chinese population, further emphasizing the role of autophagy in systemic lupus erythematosus. PMID: 24943867
- Evidence suggests that MTMR3, PIKFYVE (phosphoinositide kinase FYVE), and their product phosphatidylinositol 5-phosphate are involved in the activation of RAC1 (rho family small GTP binding protein). This process regulates migration/invasion of carcinoma/sarcoma. PMID: 24840251
- Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration. PMID: 23154468
- MTMR3 belongs to the myotubularin family of phosphoinositides phosphatases. PMID: 9736772
- MTMR3 shares similar phosphatase activity and substrate specificity with its homologous proteins MTM1 and MTMR3. PMID: 11846405
- A phylogenetic study revealed co-evolution of myotubularins with PI 3-kinase class III complex. PMID: 18774718
- Autophagosome formation was triggered by the overexpression of a dominant-negative inactive mutant of MTMR3. PMID: 20059746
- Analysis of MTMR3 phosphoinositide binding domain properties has been conducted. PMID: 15840652
Database Links
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class myotubularin subfamily
Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein.
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