mturn Antibody, FITC conjugated

Research Applications

While specific studies using this mturn-FITC conjugate are not detailed in publicly available literature, its design aligns with standard FITC-conjugated antibody applications:

• Immunofluorescence (IF): Localization of mturn protein in zebrafish tissues or embryos .

• Flow Cytometry: Quantification of mturn-expressing cells in suspension .

• Dot Blot/ELISA: Detection of mturn in lysates or purified samples .

FITC’s high quantum yield and compatibility with 488 nm laser systems make it suitable for multiplex assays when paired with fluorophores like TRITC or Cy5 .

Conjugation Methodology

The FITC conjugation process typically involves:

1. Primary Amine Targeting: FITC binds to lysine residues on the antibody via its isothiocyanate group .

2. Optimized Stoichiometry: A ratio of 40–80 µg FITC per mg antibody is standard to avoid quenching or solubility issues .

3. Post-Conjugation Purification: Removal of unreacted FITC via gel filtration or dialysis .

For this mturn antibody, the conjugation likely followed similar protocols, ensuring minimal disruption to antigen-binding sites .

Quality Control and Validation

• Specificity: Validated against recombinant zebrafish maturin protein (1-133 AA) .

• Sensitivity: FITC’s brightness allows detection at low antibody concentrations (typical dilution range: 1:100–1:1,000) .

• Cross-Reactivity: No reported cross-reactivity with human or mouse cells .

Handling Considerations

• Light Sensitivity: FITC fluorescence degrades under prolonged light exposure; store aliquots in dark conditions .

• Sodium Azide Incompatibility: Residual azide inhibits FITC conjugation; ensure its removal during buffer exchange .

Comparative Advantages