MYC2 Antibody
Description
What is the MYC2 Antibody?
MYC2 antibodies are polyclonal or monoclonal antibodies designed to specifically bind to the MYC2 protein, a basic helix-loop-helix (bHLH) leucine zipper transcription factor. These antibodies are critical for:
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Protein detection (e.g., Western blot, immunoprecipitation).
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Localization studies (e.g., immunofluorescence, immunohistochemistry).
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Functional assays (e.g., chromatin immunoprecipitation [ChIP] to identify MYC2-bound DNA regions).
MYC2 is pivotal in JA signaling, regulating genes involved in defense against herbivores, pathogens, and mechanical stress . The antibody’s specificity ensures accurate tracking of MYC2 expression, post-translational modifications (e.g., phosphorylation), and protein-protein interactions.
Key Applications
Validation Data
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Specificity: Anti-MYC2 antibodies show minimal cross-reactivity with other bHLH proteins .
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Phosphorylation Detection: Antibodies can differentiate phosphorylated MYC2 (e.g., Thr328 phosphorylation by MPK6) .
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Mutant Validation: Used to confirm MYC2 protein absence in myc2-1 and myc2-2 mutants .
Mechanistic Insights
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Phosphorylation-Dependent Degradation: MYC2 antibodies revealed that MPK6 phosphorylates MYC2 at Thr328, promoting its proteasomal degradation to terminate JA signaling .
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Transcriptional Regulation: ChIP-seq using MYC2 antibodies identified 655 MYC2-target genes, including JA biosynthesis genes (LOX2, AOS) and transcription factors (ORA59, ERF109) .
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Protein Interactions: Co-IP assays demonstrated MYC2’s interaction with DELLA proteins (e.g., RGA) and MED25, linking JA and gibberellin signaling .
Functional Studies
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In Arabidopsis, MYC2 antibodies confirmed its role in suppressing pathogen-responsive genes (PDF1.2) while activating wound-responsive genes (LOX2) .
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Tomato MYC2 (Sl-MYC2) antibodies highlighted its hierarchical role in amplifying JA-dependent defense against Botrytis cinerea .
Technical Considerations
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Antibody Validation: Ensure antibodies are tested in knockout mutants (e.g., myc2-2) to confirm specificity .
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Phosphorylation-Specific Variants: Use antibodies validated for detecting post-translational modifications critical for MYC2 stability .
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Cross-Reactivity: Verify reactivity across species (e.g., Arabidopsis, tomato) if studying non-model plants .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- The 2.7 A crystal structure of the MYC2 bHLH domain complexed with G-box DNA, revealing a cis-tetrameric structure, is reported. PMID: 28514654
- Allantoin can activate the MYC2-regulated jasmonic acid signaling pathway through abscissic acid production. PMID: 26931169
- MYC2 interacts with ANAC019 to co-regulate the expression of NYE1 during jasmonic acid-induced chlorophyll degradation. PMID: 26407000
- Under salt stress, JA exhibits a minimal positive effect on jin1 plants. This finding suggests that the protein JIN1/MYC2 plays a role in controlling protective systems under salt stress. PMID: 27266252
- MYC2 is targeted by PUB10 for degradation during jasmonic acid responses. PMID: 26163577
- MYC2 differentially regulates GATA-box containing promoters during seedling development in Arabidopsis. PMID: 23857363
- Research indicates that phosphorylation-coupled turnover of MYC2 stimulates its transcription activity. PMID: 23593022
- MYC2 regulates RGL3 expression through a direct association with its promoter. PMID: 22892320
- During jasmonate signaling, MED25 physically associates with the basic helix-loop-helix transcription factor MYC2 in promoter regions of MYC2 target genes, positively influencing MYC2-regulated gene transcription. PMID: 22822206
- MYC2 interacts with RGA proteins in regulating sesquiterpene synthase gene expression. PMID: 22669881
- Jasmonic acid predominantly promotes MYC2 protein accumulation in the morning, while TIME FOR COFFEE represses accumulation of MYC2. PMID: 22693280
- MYC2 functions as a negative regulator of light-induced gene expression. PMID: 22424472
- MYC2-mediated repression of PLT expression is implicated in jasmonate inhibition of primary root growth. PMID: 21954460
- The jasmonate-responsive activity of the jasmonate-responsive element from the ORCA3 promoter interacts in vitro and in vivo with the basic helix-loop-helix transcription factor AtMYC2. PMID: 21306988
- High- and medium-affinity binding sites are over-represented in promoters of MYC2- or ERF1 (ethylene-responsive transcription factor 1B)-regulated genes, suggesting they may represent novel cis-regulatory elements. PMID: 21284757
- The role of jasmonate and salicylic acid signaling in susceptibility to P. syringae infection in A. thaliana mutants is reported. PMID: 16838791
- These findings provide valuable insights into the function of MYC2 and the transcriptional coordination of the jasmonic acid signaling pathway. PMID: 17616737
- Research highlights MYC2 as a potential regulator in priming for enhanced JA-responsive gene expression during rhizobacteria-mediated ISR. PMID: 18657213
