Myc Antibody
Description
Definition and Core Characteristics
Myc antibodies target either:
-
Endogenous c-Myc protein: A transcription factor encoded by the MYC proto-oncogene, critical for cell proliferation, differentiation, and apoptosis .
-
Myc-tagged proteins: A 10-amino acid epitope tag (EQKLISEEDL) derived from the c-Myc protein, widely used in recombinant protein studies .
Key Antibody Clones:
Applications in Research
Myc antibodies are indispensable for:
-
Western Blotting (WB): Detecting Myc-tagged proteins (e.g., 9E10) or endogenous c-Myc (e.g., Y69) .
-
Immunohistochemistry (IHC): Mapping c-Myc expression in cancer tissues. Discrepancies exist between N-terminal (Y69) and C-terminal (9E10) antibodies, with Y69 showing better correlation with MYC mRNA levels .
-
Chromatin Immunoprecipitation (ChIP): Identifying Myc-regulated genes (e.g., CDC25A, EIF4E) .
-
Flow Cytometry: Quantifying c-Myc expression in leukemia cells .
c-Myc in Cancer:
-
Oncogenic Role: Drives tumorigenesis by regulating ~15% of human genes, including cell cycle and metabolism regulators .
-
Therapeutic Targeting: Suppressing c-Myc induces tumor regression in preclinical models (e.g., lung adenocarcinoma, lymphoma) .
Antibody Validation Highlights:
Technical Considerations
Future Directions
Product Specs
Product Science Overview
Myc is a family of regulator genes and proto-oncogenes that code for transcription factors. These transcription factors play a crucial role in cell cycle progression, apoptosis, and cellular transformation. The Myc family includes c-Myc, N-Myc, and L-Myc, with c-Myc being the most studied due to its significant role in various cancers.
The Myc protein functions as a transcription factor that regulates the expression of numerous genes involved in cell growth and proliferation. It contains a basic helix-loop-helix leucine zipper (bHLH-ZIP) motif, which is essential for DNA binding and dimerization with other proteins such as Max. The Myc/Max complex binds to E-box sequences in the promoter regions of target genes, activating their transcription.
Polyclonal antibodies are produced by immunizing animals with an antigen, in this case, a synthetic peptide corresponding to amino-terminal residues of the Myc protein. The immune system of the animal generates a diverse array of antibodies that recognize multiple epitopes on the antigen. These antibodies are then harvested and purified for use in various research applications.
The production of Myc polyclonal antibodies involves several steps:
- Immunization: Animals, typically rabbits, are immunized with a synthetic peptide derived from the Myc protein.
- Antibody Generation: The animal’s immune system produces antibodies against the Myc peptide.
- Harvesting: Blood is collected from the immunized animals, and the serum, which contains the antibodies, is separated.
- Purification: The antibodies are purified using protein A and peptide affinity chromatography to ensure high specificity and purity.
Myc polyclonal antibodies are widely used in various research applications, including:
- Western Blotting: To detect Myc protein levels in cell lysates.
- Immunoprecipitation: To isolate Myc protein complexes from cell extracts.
- Chromatin Immunoprecipitation (ChIP): To study Myc binding to DNA in the context of chromatin.
- Immunofluorescence: To visualize Myc protein localization within cells.
The Myc protein is implicated in numerous cellular processes, and its dysregulation is associated with various cancers, including Burkitt lymphoma, leukemia, and other hematopoietic tumors. Understanding the role of Myc in these diseases is crucial for developing targeted therapies. Polyclonal antibodies against Myc provide researchers with valuable tools to study the protein’s function, regulation, and involvement in cancer.
