MYH2 Antibody, FITC conjugated
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Description
Key Applications
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Muscle Fiber Typing: Distinguishes fast-twitch (Type IIA) skeletal muscle fibers in human and rodent tissues .
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Myogenesis Studies: Tracks MYH2 expression during muscle differentiation in vitro (e.g., C2C12 myotubes) .
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Disease Biomarker Analysis: Investigates MYH2 aberrations in myopathies and inclusion body diseases .
Validation Data
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Specificity: Confirmed via Western blot (WB) and IHC in skeletal muscle lysates; no cross-reactivity with MYH1 or MYH3 isoforms .
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Sensitivity: Detects MYH2 at dilutions up to 1:500 in IHC (human tissue) and 1:2,000 in WB .
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Performance in Multiplex Assays: Compatible with multi-color fluorescence setups (e.g., combined with DAPI and Cy5-labeled antibodies) .
Recommended Protocols
| Method | Dilution Range | Buffer/Retrieval |
|---|---|---|
| Immunofluorescence | 1:50–1:200 | PBS with 1% BSA; antigen retrieval (pH 9.0 TE buffer) |
| Western Blot | 1:1,000–1:5,000 | Tris-glycine SDS-PAGE, 5% non-fat milk |
Critical Controls
Comparative Analysis of MYH2 Antibody Conjugates
Research Findings Using FITC-Conjugated MYH2 Antibodies
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Muscle Atrophy Studies: Demonstrated reduced MYH2 expression in disuse-atrophied mouse muscle, correlating with fiber-type shifts .
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Hybrid Fiber Detection: Enabled identification of MYH IIA/IIX hybrid fibers in human biopsies via co-staining with SC-71 (MyHC IIA) .
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Drug Screening: Quantified MYH2 restoration in myotubes treated with trimetazidine, a mitochondrial enhancer .
Supplier Landscape and Citations
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Leading Suppliers: BosterBio (Catalog #MA1063), Bioss Inc. (FITC-conjugated), antibodies-online.com .
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Citations: Over 600 studies cite MYH2 antibodies, including muscle regeneration and metabolic research .
Limitations and Troubleshooting
Product Specs
Buffer
**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
We typically dispatch orders within 1-3 business days of receipt. Delivery timelines may vary depending on the purchasing method and location. Please consult your local distributors for specific delivery estimates.
Synonyms
adult 2 antibody; Fast 2a myosin heavy chain antibody; IBM3 antibody; Inclusion body myopathy 3, autosomal dominant antibody; MYH2 antibody; MYH2_HUMAN antibody; MYH2A antibody; MYHas8 antibody; MyHC IIa antibody; MyHC-2a antibody; MyHC-IIa antibody; MYHSA2 antibody; Myosin heavy chain 2 antibody; Myosin heavy chain 2a antibody; Myosin heavy chain antibody; Myosin heavy chain IIa antibody; Myosin heavy chain skeletal muscle adult 2 antibody; Myosin heavy polypeptide 2 skeletal muscle adult antibody; Myosin-2 antibody; MYPOP antibody; skeletal muscle antibody; Type IIA myosin heavy chain antibody
Target Names
MYH2
Uniprot No.
Target Background
Function
MYH2 (Myosin Heavy Chain 2) is a protein involved in muscle contraction and essential for maintaining cytoskeleton organization.
Gene References Into Functions
- Exome analysis revealed homozygosity for a novel truncating mutation p.G800fs27* in the MYH2 gene in both brothers, while parents and an unaffected sibling were heterozygous. PMID: 28729039
- The study demonstrated that the differential regulation of PKA and cell stiffness in unconfined versus confined cells is abrogated by dual, but not individual, inhibition of Piezo1 and myosin II. PMID: 27160899
- C-terminal Myosin IIA Heavy Chain phosphorylation sites are critical for recruitment of Myosin IIA to lamellar protrusions and for marginal paxillin phosphorylation during active cell spreading. PMID: 28053086
- Research revealed that five of the patients were homozygous for myosin heavy chain 2 (MYH2) missense mutations, one patient was compound heterozygous for a missense and a nonsense mutation, and one patient was homozygous for a frame-shift MYH2 mutation. PMID: 24193343
- Findings indicate that a greater MyH2 content in the vastus lateralis is accompanied by a higher oxygen cost of cycling during exercise performed below the lactate threshold. PMID: 24781731
- This study presented more cases of MYH2 mutation in recessive myopathy with external ophthalmoplegia linked to chromosome 17p13.1-p12. PMID: 23388406
- A previously unrecognized interplay between actin and myosin IIA in podosomes was demonstrated. PMID: 23361003
- Myosin heavy chain 2A transcripts decreased significantly in skeletal muscle tissue from overnight parenterally fed patients but did not change significantly in orally refed mice. PMID: 23190566
- Phenotypic expression of alpha-smooth muscle actin, smooth muscle myosin heavy chain 2, and smoothelin were significantly decreased in the dissected media, whereas that of osteopontin was elevated. PMID: 22960022
- This study demonstrated the missense mutation c.2542T>C (p.V805A) in the MYHC2A gene. PMID: 22349865
- The human genioglossus muscle is composed of conventional myosin heavy chain isoforms and 3 primary myosin hevy chain phenotypes. PMID: 22337492
- NMMII and actin isoform expression changes coordinately with the remodeling phase of repair, and NMMII is increased as matrix stiffness increases. As NMMII expression increases, so does the fibroblast contractility. PMID: 21102503
- Null mutations in the fast myosin heavy chain IIa gene cause early onset myopathy and demonstrate that this isoform is necessary for normal muscle development and function. PMID: 20418530
- The study investigated the relation between expression of the mutant MyHC IIa and pathologic changes in muscle. PMID: 11889243
- Data suggest that changes in intracellular calcium may play a role in shifts in myosin heavy chain IIa (MyHC IIa) expression during muscle activation. PMID: 12235157
- IRF-2 is involved in up-regulation of nonmuscle myosin heavy chain II-A gene expression in cell differentiation. PMID: 15496418
- Analysis of normal variation indicates that there is strong selective pressure against mutations in MYH2; On the basis of these results, we suggest that MyHC genes should be regarded as candidate genes in hereditary myopathies of unknown etiology. PMID: 15741996
- Our results provide evidence that the pathogenesis of the MyHC IIa E706K myopathy involves defective function of the mutated myosin as well as alterations in the structural integrity of all muscle cells irrespective of MyHC isoform expression. PMID: 17005402
- Myosin II has a role in glioma invasion of the brain. PMID: 18495866
Database Links
Involvement In Disease
Myopathy, proximal, and ophthalmoplegia (MYPOP)
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
Subcellular Location
Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
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