MYO9B Antibody
Description
Introduction to MYO9B Antibody
MYO9B antibodies are immunological reagents designed to detect and quantify MYO9B protein expression in experimental models. MYO9B is an actin-based molecular motor with RhoGAP activity, critical for regulating RhoA signaling, cell motility, and immune responses. These antibodies enable researchers to explore MYO9B's role in autoimmune diseases, cancer, and metabolic disorders .
Key Features of MYO9B Antibodies
Commercial MYO9B antibodies vary in reactivity, applications, and validation. Below is a comparative table of widely used antibodies:
| Product | Host | Applications | Reactivity | Molecular Weight | UniProt ID |
|---|---|---|---|---|---|
| DF4199 (Affinity) | Rabbit | WB, IF/ICC | Human, Mouse, Rat | ~250 kDa | Q13459 |
| 12432-1-AP (Proteintech) | Rabbit | WB, IHC, IF, IP, ELISA | Human, Mouse, Rat | 230–250 kDa | Q13459 |
| ab243451 (Abcam) | Rabbit | IHC-P, ICC/IF | Human | Not specified | Q13459 |
WB = Western blot; IF/ICC = Immunofluorescence/Immunocytochemistry; IHC = Immunohistochemistry; IP = Immunoprecipitation.
Autoimmune Diabetes and Dendritic Cell Regulation
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MYO9B mutations (e.g., a 33-bp deletion in mice and R133Q polymorphism in humans) alter dendritic cell (DC) maturation and suppress autoimmune diabetes progression .
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Knock-in (KI) and knockout (KO) mouse models show reduced pancreatic islet infiltration, decreased proinflammatory cytokines (IL-1β, TNF-α), and increased regulatory T-cell (Treg) activity .
Cancer Metastasis
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MYO9B overexpression in lung cancer correlates with poor prognosis. It interacts with ROBO receptors to modulate the SLIT/ROBO/Myo9b/RhoA pathway, inhibiting tumor cell invasion and metastasis .
Osteocyte Function
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MYO9B regulates osteocyte dendrite formation and mechanotransduction. Knockdown experiments in bone cells reveal impaired responses to mechanical stress .
Molecular Mechanisms and Pathways
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RhoA Regulation: MYO9B inactivates RhoA via its RhoGAP domain, influencing cell migration and cytoskeletal organization .
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Metabolic Signaling: In DCs, MYO9B modulates PTEN/Akt pathways and glycolytic metabolism, affecting immune activation .
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Immune Interactions: MYO9B associates with TGF-β signaling components, impacting intestinal barrier function and gut immunity .
Clinical and Therapeutic Implications
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Autoimmunity: MYO9B variants are linked to type 1 diabetes (T1D), lupus, and rheumatoid arthritis, highlighting its potential as a therapeutic target .
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Cancer: Targeting the SLIT/ROBO/Myo9b axis may offer strategies to inhibit metastasis .
Future Directions
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Biomarker Development: Correlating MYO9B expression levels with disease progression in patient cohorts.
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Mechanistic Studies: Elucidating MYO9B’s role in cross-talk between metabolic and immune pathways.
Product Specs
Target Background
- Analysis of PTPN22, ZFAT and MYO9B polymorphisms in Turner Syndrome and risk of autoimmune disease. PMID: 28627089
- Data indicate that polymorphisms in MY09B are associated with the risk of inflammatory bowel disease PMID: 27556856
- Mutating either of the two arginine fingers impaired the catalytic activity of Myo9b-RhoGAP. PMID: 27363609
- Meta-analysis indicates that MYO9B gene polymorphisms might not be associated with celiac disease susceptibility in Caucasians. [meta-analysis] PMID: 27219348
- This meta-analysis shows that MYO9B genetic polymorphism is associated with Crohn's disease and ulcerative colitis PMID: 27435931
- Newly defined SLIT/ROBO/Myo9b/RhoA signaling pathway that restricts lung cancer progression and metastasis. PMID: 26529257
- Our data suggest a link between MYO9B variants to an increased intestinal permeability in Crohn's disease patients. PMID: 25098938
- Results do not support the association of MYO9B with schizophrenia in the Chinese population. PMID: 25710847
- MYO9B SNPs may influence the sub-phenotypic expression of Crohn's disease but did not find an association between these MYO9B polymorphisms and intestinal permeability in IBD. PMID: 24966617
- Variants in MYO9B may be involved in acute pancreatitis PMID: 24386489
- Genetic variation in the MYO9B gene is associated with celiac disease as a protective or a risk factor PMID: 23368647
- The homozygous G/G group of the Myo9B polymorphism was associated with an increased risk for Barrett's esophagus and esophageal adenocarcinomas (EAC) development. Also, the heterozygous A/G genotype was associated with an increased risk for EAC development. PMID: 22954106
- Critical roles for Myo9b during epithelial wound healing and maintenance of tight junction integrity - key functions that may be altered in patients with Myo9b-linked inflammatory bowel disease. PMID: 22573889
- We performed genetic analysis of the MYO9B gene and the IL-2/IL-21 locus by genotyping SNPs that have been previously associated with celiac disease or schizophrenia, and found no evidence for association with these two loci. PMID: 21688385
- These data demonstrate an association of MYO9B with ileal CD. PMID: 21515326
- Gene polymorphism is associated with type 1 diabetes in the Dutch but not in the Brotosh population PMID: 20303373
- Myosin-IXb is a single-headed and processive motor PMID: 11801597
- Myosin IXb binds to BIG1, which regulates its Rho-GTPase activating protein activity PMID: 15644318
- Individuals homozygous with respect to the at-risk allele have a 2.3-times higher risk of celiac disease (P = 1.55 x 10(-5)). PMID: 16282976
- Myosin IX uses a unique ATP hydrolysis mechanism PMID: 16338935
- Genotyping of the three SNPs which tagged the associated haplotype was performed in a Celiac disease family dataset in a Swedish/Norwegian cohort. PMID: 16720215
- Tested the association between celiac disease and the three most associated single nucleotide polymorphisms by the transmission disequilibrium test in the Italian population PMID: 16943798
- Unlike previous variants (in other genes) reported to predispose to inflammatory bowel disease, the association at MYO9B was considerably stronger with ulcerative colitis, although weaker association with Crohn's disease also was observed. PMID: 17087940
- Results support a negligible influence of MYO9B polymorphisms on celiac disease predisposition. PMID: 17176439
- The MYO9B gene rs 2305764 polymorphism is not associated to coeliac disease in coeliac children from southern Italy. The allelic frequencies of the polymorphism found in these patients and in the population control were not statistically different. PMID: 17267307
- Study suggests that genetic variation in MYO9B is associated with celiac disease, systemic lupus erythematosus, and rheumatoid arthritis and that MYO9B is a general risk factor for autoimmunity PMID: 17584584
- Our data and the results of our meta-analysis question the role of MYO9B as a causative gene for celiac disease. PMID: 17667713
- Study did not confirm the association of celiac disease with the CELIAC4 region polymorphisms described in other populations PMID: 17767555
- Demonstrate significant association of allelic variants in MYO9B with schizophrenia. To our knowledge, this is the first molecular genetic evidence for a correlation between autoimmune diseases and the risk of developing schizophrenia PMID: 17948900
- MYO9B homozygosity might be involved in the prognosis of CD and the chance of developing RCD II and EATL. PMID: 17967566
- Myosin IXB variants were not associated with coeliac disease in this study; however, weak evidence of association with dermatitis herpetiformis was found. PMID: 18077767
- Minor alleles of rs962917, rs2279003, and rs2305764 polymorphisms were more frequent in diabetic patients than in controls, and the haplotype carrying major alleles in rs962917*G/rs2279003*C/rs2305764*G, significantly reduced the risk of type 1 diabetes PMID: 18361936
- No association of MYO9B variants to multiple sclerosis (MS) in four Northern European populations PMID: 19142207
- MYO9B variants may be involved in inflammatory bowel disease pathogenesis PMID: 19235913
