N Antibody, Biotin conjugated

ELISA and Immunoassays

• Used as a capture or detection antibody in sandwich ELISA for NP quantification .

• Biotin conjugation enables signal amplification via streptavidin-HRP or fluorophore-linked systems .

Viral Research

• Detects NP in infected cells to study viral replication dynamics .

• Supports epitope mapping for vaccine development .

Clinical Diagnostics

• Potential utility in rapid lateral flow assays (LFAs) for influenza A detection, as demonstrated with biotinylated nanobodies .

Production and Conjugation

• Labeling Method: Utilizes kits like Biotium’s Mix-n-Stain™, which biotinylates antibodies in 15 minutes without purification .

• Degree of Labeling: Typically 4–6 biotin molecules per antibody, optimized for high-affinity binding .

Key Considerations:

• Site-specific conjugation (e.g., N-terminal) minimizes epitope interference, critical for ADA assays .

• Compatibility with buffers containing BSA or ascites .

Research Findings and Optimization

• Biotin Positioning: Studies show that biotin placement within antibody sequences significantly impacts binding efficiency .

• Stability: Biotinylated antibodies retain activity for >1 year at -20°C .

• Cross-Reactivity: No reported cross-reactivity with other viral nucleocapsid proteins .

Table: Application-Specific Performance

Future Directions

• Therapeutic Monitoring: Integration into anti-drug antibody (ADA) assays for biologics targeting NP .

• Multiplexing: Combining with fluorescently labeled antibodies for multianalyte detection .