NANOS3 Antibody
Description
Introduction to NANOS3 Antibody
NANOS3 antibody is a specialized immunological tool designed to detect and study the NANOS3 protein, a conserved RNA-binding protein critical for germ cell development across species. This antibody enables researchers to investigate NANOS3's roles in maintaining pluripotency, regulating meiosis, and preventing germ cell apoptosis . Its applications span western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA, with validated reactivity in human, mouse, and rat samples .
Antibody Characteristics
Key specifications of the NANOS3 antibody (Catalog No. 21679-1-AP, Proteintech) include:
| Property | Details |
|---|---|
| Host/Isotype | Rabbit IgG |
| Immunogen | NANOS3 fusion protein (Ag16014) |
| Observed MW | 26–30 kDa (vs. calculated 21 kDa due to post-translational modifications) |
| Tested Reactivity | Human, mouse, rat |
| Applications | WB (1:200–1:1000), IHC (1:50–1:500), IF, ELISA |
| Storage | -20°C in PBS with 0.02% sodium azide and 50% glycerol |
This antibody detects nuclear and cytoplasmic NANOS3 localization, particularly in mitotic/meiotic germ cells .
Germ Cell Development Studies
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Role in Germ Cell Survival: Knockdown of NANOS3 using morpholinos reduced human germ cell numbers by 33% (p < 0.05) and downregulated DAZL expression by 50% .
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Cell Cycle Regulation: NANOS3 modulates the G2/M phase transition by repressing cell cycle targets like CCNB1 and CDK1, confirmed via RNA-sequencing in TCam-2 cells .
CRISPR/Cas9 Validation
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Germline Ablation: Bovine NANOS3 knockout via CRISPR/Cas9 caused complete germ cell loss in 75% of edited embryos, with testis weight reduced by 40% in edited fetuses .
Disease Associations
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Premature Ovarian Insufficiency (POI): The NANOS3 Arg153Trp mutation accelerates protein degradation, correlating with reduced primordial germ cell (PGC) pools in mice .
Antibody Specificity
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Western Blot: Detects a single band at 26–30 kDa in human testis lysates .
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Immunofluorescence: Co-localizes with chromosomal DNA in human germ cell nuclei during mitosis/meiosis .
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IHC Optimization: Antigen retrieval with TE buffer (pH 9.0) enhances signal in human testis sections .
Knockdown Efficiency
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Morpholino Studies: Achieved 50% NANOS3 protein reduction at day 0, recovering to baseline by day 7 .
Functional Insights from Key Studies
Limitations and Considerations
Product Specs
Target Background
- Despite the significant role of NANOS3 in ovarian development, our findings indicate that NANOS3 mutations were not associated with premature ovarian failure (POF) in the studied cohort of Brazilian women. PMID: 28076512
- This data can be further utilized to elucidate the role of NANOS3 and DAZL in germ cell development, both in vitro and in vivo. PMID: 27768780
- NANOS3 contributes to the acquisition of invasiveness by human lung tumor cells and proposes a novel mechanism of post-transcriptional regulation of epithelial-mesenchymal transition. PMID: 25904364
- An inactivating missense mutation in NANOS3 plays a role in primary ovarian insufficiency, involving increased primordial germ cell apoptosis during embryonic cell migration. PMID: 25054146
- A NANOS3 mutation is a potential cause of primary ovarian insufficiency. PMID: 24091668
- NANOS3 modulates key aspects of human germ cell development, potentially during the cell cycle. PMID: 21421998
- Mutations in NANOS3 exons are uncommon in both Chinese and Caucasian women with premature ovarian failure. PMID: 17418157
- This report presents the first analysis of the NANOS3 gene in infertile males. PMID: 19565640
- Observational study of genotype prevalence. (HuGE Navigator) PMID: 17418157
