NAP1L1 Antibody
Description
Applications in Research
NAP1L1 antibodies are widely used for:
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Western Blot (WB): Detects endogenous NAP1L1 at ~55–60 kDa in cell lines (e.g., HeLa, Jurkat) and tissues (e.g., mouse spleen) .
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Immunofluorescence (IF): Localizes NAP1L1 in cytoplasmic and peri-nuclear regions of megakaryocytes and platelets .
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Functional Studies:
Molecular Functions of NAP1L1
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Chromatin Dynamics: Facilitates nucleosome assembly by shuttling histones H2A-H2B into the nucleus .
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DNA Repair: Enhances ERCC6-mediated chromatin remodeling for nucleotide excision repair and RAD51/RAD54-dependent homologous recombination .
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Cell Cycle Regulation: Promotes G2/M phase transition in HCC by upregulating CDK1 and β-catenin .
Role in Cardiac Fibrosis
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Mechanism: NAP1L1 binds YAP1, stabilizing it to drive cardiac fibroblast proliferation and differentiation into myofibroblasts .
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In Vivo Impact: NAP1L1 knockout mice showed reduced fibrosis post-myocardial infarction and improved cardiac function .
Oncogenic Implications in Hepatocellular Carcinoma
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Prognostic Marker: High NAP1L1 expression correlates with poor survival (HR = 1.54, p < 0.001) and promotes HCC progression via Wnt/β-catenin activation .
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Therapeutic Target: Silencing NAP1L1 reduces CDK1 and β-catenin levels, arresting cells in the G2/M phase .
Role in Platelet Biology and Sepsis
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Mitochondrial Interaction: Binds PDH complex subunit PDC-E2 (DLAT), modulating PDH activity in platelets .
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Clinical Relevance: Elevated NAP1L1 and PDH activity observed in platelets from septic patients, suggesting metabolic reprogramming during infection .
Future Directions
Product Specs
Target Background
- In a recent study, researchers identified NAP1L1 as a novel binding partner of hepatitis C virus NS3. They found that the protease domain of NS3 is responsible for interacting with NAP1L1. The study demonstrated the functional role of NAP1L1 in hepatitis C virus entry through regulating the protein expression of SR-B1. PMID: 29541944
- Knockdown of NAP1L1 suppresses IkappaBalpha degradation and nuclear transport of the p65 subunit after treatment with TNF-a stimulation, leading to attenuation of NF-kappaB transcriptional activity. Conversely, NAP1L4 knockdown remains inactive. The study suggests that NAP1L1 downregulation makes cells vulnerable to apoptotic cell death due to attenuation of NF-kappaB transcriptional activity on anti-apoptotic genes. PMID: 28687276
- NAP1L1 increases CSB processivity by decreasing the pausing probability during translocation. This study sheds light on different steps of CSB-mediated chromatin remodeling regulated by NAP1L1. PMID: 28369616
- This study confirmed the hepatitis C virus NS5A-NAP1L1 interaction and mapped it to the C terminus of NS5A in both hepatitis C virus genotype 1 and 2. PMID: 28659470
- Nap1 binds to RAD54. PMID: 24798879
- TAF-Ibeta promotes BZLF1 expression and subsequent lytic infection by influencing chromatin at the BZLF1 promoter. PMID: 23691099
- NAP1L1 was downregulated following antiproliferative agent treatment in AuL12-treated cells compared to control and Au(2)Phen-treated ovarian cancer cells. PMID: 22134777
- This study identified several proteins interacting with NAP1L2, including the ubiquitously expressed members of the nucleosome assembly protein family, NAP1L1 and NAP1L4. PMID: 21333655
- The biochemical properties of two human NAP1-like proteins, hNAP1L1 and hNAP1L4, were characterized. PMID: 20002496
- These findings suggest that the binding of sequence-specific DNA binding proteins to human nucleosome assembly protein 1 may be a crucial step in the activation of transcription. PMID: 14966293
- Both the acidic nature of nucleosome assembly protein I and its other functional structures may be essential for mediating the assembly and disassembly of dimers in nucleosome core particles. PMID: 16287874
- Data show that Alien binds in vivo and in vitro to NAP1 and modulates its activity by enhancing NAP1-mediated nucleosome assembly on DNA. PMID: 17339334
