NAC100 Antibody
Description
Clarification of Terminology
The term "NAC" has distinct meanings depending on context:
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N-Acetylcysteine (NAC): A small-molecule antioxidant used clinically for mucolytic and detoxification purposes .
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NAC1 Protein: A transcriptional repressor (Nucleus Accumbens Associated 1, NACC1) implicated in carcinomas .
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NAC Antibodies: Targeting proteins like the X-linked Kx blood group antigen (XK gene product) or NAC1 .
No antibody named "NAC100" appears in commercial catalogs or research databases. Potential interpretations include:
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Typographical Error: Possible confusion with "NAC1 Antibody" (e.g., NB100-58964 from Bio-Techne) .
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Proprietary Designation: A non-standard identifier for an in-house antibody.
Analysis of NAC1 Antibody (NB100-58964)
The closest match to "NAC100" is NAC1 Antibody (NB100-58964), a polyclonal goat IgG antibody validated for human NAC1 protein detection .
| Property | Specification |
|---|---|
| Target | NAC1 protein (Nucleus Accumbens Associated 1) |
| Immunogen | Peptide sequence: KTEQQESDSVQC (Internal region of NP_443108.1) |
| Applications | Western Blot, Peptide ELISA |
| Reactivity | Human (validated); Canine predicted (100% sequence homology) |
| Concentration | 0.5 mg/ml in Tris saline with 0.5% BSA and 0.02% sodium azide |
| Observed Band | ~55 kDa in human bone marrow lysates (calculated MW: 57.3 kDa) |
Research Findings:
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Western Blot: Detects a single band at ~55 kDa in human bone marrow lysates .
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Biological Role: NAC1 is upregulated in carcinomas and acts as a transcriptional repressor with a BTB domain .
Other NAC-Targeted Antibodies
Additional antibodies targeting NAC-related proteins include:
| Antibody Name | Target Protein | Applications | Supplier |
|---|---|---|---|
| Anti-NAC-1 (CBP3951) | NAC1 (NACC1) | WB, FCM, ICC, IHC-p | Creative Biolabs |
| Purified anti-NAC-1 | NAC1 | WB, ELISA | BioLegend (849302) |
| Anti-NACAD Antibody | NACAD (Nucleus Accumbens Associated Domain) | WB | BosterBio |
Key Observations:
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NAC1 Antibodies: Primarily used in cancer research to study transcriptional regulation .
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Anti-NAC (XK Protein): Targets the X-linked Kx blood group antigen, with applications in hematology .
N-Acetylcysteine (NAC) and Antibody Modulation
While not an antibody, NAC (the compound) influences antibody production:
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Mechanism: Downregulates CD40/CD27 co-stimulatory molecules on B cells, reducing T-dependent antibody responses to antigens like Candida albicans .
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Cytokine Effects: Suppresses IL-4 (Th2 cytokine) and elevates IFN-γ (Th1 cytokine), promoting a Th1-polarized response .
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Clinical Relevance: In SLE patients, NAC reduces anti-phospholipid antibody levels (e.g., anti-β2GPI) when combined with rapamycin .
Recommendations for Further Inquiry
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Verify Nomenclature: Confirm whether "NAC100" refers to a specific antibody clone, lot number, or proprietary designation.
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Explore NAC1 Antibodies: For studies involving NAC1 protein, prioritize validated reagents like NB100-58964 .
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Consult Supplier Databases: Use platforms like Biocompare to compare NAC-targeting antibodies across suppliers .
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
