NCAPG2 Antibody

Overview of NCAPG2 Antibody

The NCAPG2 antibody (e.g., NBP1-86633 from Novus Biologicals/Bio-Techne) is a monoclonal antibody validated for detecting human NCAPG2 protein in research applications. It targets the condensin II complex subunit involved in mitosis and chromosomal stability .

Key Features:

• Applications: Validated for immunocytochemistry (ICC), immunofluorescence (IF), and immunohistochemistry (IHC) on paraffin-embedded tissues .

• Specificity: Shows nuclear speckle localization in human A-431 cells and moderate-to-strong nuclear staining in tonsil germinal centers and testis seminiferous ducts .

Table 2: NCAPG2 Expression in Cancer Studies

Mechanistic Insights:

• Immune Regulation: NCAPG2 expression positively correlates with immune checkpoint genes (PDCD1, PDCD1LG2, LAG3) and immune cell infiltration (macrophages, dendritic cells) .

• Therapeutic Potential: High NCAPG2 levels predict resistance to immunotherapy in LGG and pancreatic cancer .

Technical Considerations for Use

• Sample Preparation: HIER (heat-induced epitope retrieval) at pH 6 recommended for IHC-Paraffin .

• Validation: Western blotting and RT-PCR confirmed knockdown efficiency (>80%) in functional studies .

Limitations and Future Directions

• Current studies rely on TCGA database analyses; prospective clinical trials are needed .

• In vivo models are required to validate NCAPG2's role in tumor microenvironment regulation .