ncbp3 Antibody
Description
Key Features:
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Subcellular Localization: Primarily nuclear and cytoplasmic .
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Species Reactivity: Broad conservation across mammals (mouse, rat, bovine, human) and non-mammals (zebrafish, chicken) .
Applications of NCBP3 Antibody
NCBP3 antibodies are widely used in:
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Western Blotting: Detecting endogenous NCBP3 expression in cell lysates .
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Immunoprecipitation (IP): Studying protein interactions (e.g., with METTL3, eIF4A2, or exon junction complex components) .
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Functional Studies: Investigating roles in viral defense, hypoxia responses, and RNA metabolism .
Table 1: Key Discoveries Using NCBP3 Antibodies
Table 2: Antibody Performance Data
| Parameter | Detail |
|---|---|
| Target Epitope | Full-length human NCBP3 (UniProt ID: Q53F19) |
| Host Species | Rabbit (common) |
| Applications | Western Blot (primary), IP, immunofluorescence |
| Cross-Reactivity | Human, mouse, rat, zebrafish |
| Buffer | PBS with 0.02% sodium azide |
Biological Significance of NCBP3
NCBP3 antibodies have illuminated its non-redundant roles in:
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Viral Infections: NCBP3 ensures cytokine mRNA translation, critical for antiviral immunity .
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Hypoxic Stress: Facilitates METTL3-mediated m6A methylation to enhance cardiac gene translation .
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Cancer and Disease: Linked to mRNA export defects in transformed cells and viral susceptibility .
Challenges and Future Directions
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Functional Redundancy: NCBP3 partially overlaps with NCBP2 in mRNA export, complicating phenotype interpretation .
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Context-Dependent Roles: NCBP3’s impact on translation varies by cell type and stress condition .
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Therapeutic Potential: Targeting NCBP3 pathways could modulate antiviral responses or hypoxia adaptation in disease .
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
