NCED6 Antibody

Definition and Biological Relevance of NCED6

NCED6 is a seed-specific NCED gene in Arabidopsis thaliana that contributes to ABA biosynthesis during seed maturation. Unlike NCED3, which is stress-induced, NCED6 is primarily expressed in the endosperm and embryo during seed development . Mutations in nced6 reduce ABA levels, leading to altered seed dormancy and resistance to paclobutrazol, a gibberellin biosynthesis inhibitor . Overexpression of NCED6 increases ABA accumulation, underscoring its regulatory role .

Applications in Research

NCED6 antibodies enable precise analysis of protein localization, expression, and post-translational modifications. Below are key applications supported by indirect evidence from NCED family studies:

Role in Seed Dormancy

• NCED6 and NCED9 are critical for ABA synthesis during seed maturation. Double mutants (nced6 nced9) show reduced dormancy, while overexpression delays germination .

• Chromatin Silencing: RZ-1 and PRC2 cooperatively silence NCED6 during germination to suppress ABA production, enabling seedling establishment .

Stress and Developmental Regulation

• ABA Biosynthesis: NCED6 contributes to ABA accumulation in the endosperm, which signals to the embryo to maintain dormancy .

• Transcriptional Control: MYB96 and DREB2C activate NCED6 in response to dehydration, while bHLH57 regulates NCED9 .

Comparative Insights from NCED3

• Post-Translational Processing: NCED3 undergoes proteolytic cleavage to form active fragments; NCED6 may share similar regulatory mechanisms .

• Natural Variation: Polymorphisms in NCED3 affect enzyme activity and ABA levels, suggesting potential functional diversity in NCED6 .

Challenges and Future Directions

• Specificity Concerns: Cross-reactivity with other NCED isoforms (e.g., NCED3, NCED9) must be addressed via rigorous validation .

• Quantification: ELISA or LC-MS/MS could complement immunoblotting for precise ABA and NCED6 quantification .

• Functional Studies: CRISPR-edited NCED6 mutants paired with antibody-based assays could elucidate its role in stress resilience .

References

1. Progressive chromatin silencing of ABA biosynthesis gene permits...

2. Induction of 9-cis-epoxycarotenoid dioxygenase in Arabidopsis...

3. Functional analysis of Arabidopsis NCED6 and NCED9 genes...

4. Functional analysis of Arabidopsis NCED6 and NCED9 genes...

5. Natural Variation in 9-Cis-Epoxycartenoid Dioxygenase 3 and ABA...

6. Natural Variation in 9-Cis-Epoxycartenoid Dioxygenase 3 and ABA...

7. REVERSAL OF RDO5 1, a Homolog of Rice Seed Dormancy4...

8. Unveiling the crucial roles of abscisic acid in plant physiology

9. Progressive chromatin silencing of ABA biosynthesis gene...

10. High Temperature-Induced Abscisic Acid Biosynthesis and Its Role...