NCF2 Antibody, HRP conjugated

1. We conducted an analysis of clinical and laboratory findings of CGD with mutations in the NCF2 gene within our cohort of CGD patients. Two cases exhibited a homozygous mutation (c.835_836delAC, p.T279fsX294), representing a deletion in the NCF2 gene. A third case revealed two heterozygous mutations, IVS13-2A>T on one allele and c.1099C>T (p.) on the other allele. PMID: 28035544
2. All investigated patients presented with the same mutation (c.257 + 2T > C) in the NCF2 gene. Our research demonstrates that this mutation leads to a significant decrease in p67phox mRNA, resulting in the skipping of exon 3, which is detected in the limited amount of residual mRNA. PMID: 27220316
3. Phosphoinositol 3-phosphate plays a crucial role in regulating reactive oxygen species production by maintaining p40phox and p67phox at the phagosomal membrane. PMID: 28096301
4. The TLR4- and TLR2-induced IRAK-ERK pathway interacts with p67phox-Nox-2 for reactive oxygen species generation. This interaction regulates IL-1beta transcription and processing in monocytic cells. PMID: 26320741
5. Skeletal muscle protein expression of the NADPH oxidase subunits p22(phox), p47(phox), and p67(phox) was elevated in obese individuals compared to lean individuals. Exercise training in obese subjects led to a reduction in p22(phox) and p67(phox) expression. PMID: 27765769
6. A novel homozygous mutation was identified within the NCF2 gene. PMID: 26272171
7. Research findings indicate a critical role of the Cys-Gly-Cys triad in Nox2 for binding p67(phox). This interaction is supported by an additional binding region located C-terminal to Cys-Gly-Cys. These two regions engage with distinct partner sites in p67(phox). PMID: 26160850
8. This model highlights the central role of Arg-395 in the structure and stability of the quaternary NCF2/NCF4/VAV1/RAC1 NADPH oxidase complex. PMID: 25795782
9. Data suggest that arachidonic acid induces a direct interaction between Rac-GTP-bound p67(phox) and the C-terminal cytosolic region of phagocyte NADPH oxidase Nox2. PMID: 25056956
10. Four novel mutations in the NCF1, NCF2, and CYBB genes have been identified in chronic granulomatous disease patients in Morocco. PMID: 24596025
11. Our findings not only establish allelic heterogeneity within NCF2 associated with SLE but also emphasize the importance of multi-ethnic cohorts for identifying predisposing variants that explain additional phenotypic variations in complex diseases such as SLE. PMID: 24163247
12. NCF2 in Asian populations demonstrates a pattern of diversity characterized by a distinct haplotype structure. PMID: 23821607
13. These results provide insight into the redox-sensitive signaling mechanism that mediates cell survival involving p53 and its novel target NCF2/p67phox. PMID: 23187810
14. Case Report: this report describes defects in NCF-2, the gene encoding p67-phox, in four cases of chronic granulomatous disease. PMID: 23264412
15. Eight novel mutations in the CYBB and NCF2 genes were identified in patients with chronic granulomatous disease. PMID: 22562447
16. This variant reduced the binding of the NCF2 gene product p67(phox) to RAC2. This study identified a novel genetic association of RAC2 with Crohn's disease (CD) and confirmed the previously established association of NCF4 with ileal CD. PMID: 21900546
17. NCF2 is strongly associated with an increased risk of both childhood- and adult-onset systemic lupus erythematosus due to a single nonsynonymous coding mutation (H389Q) in exon 12. PMID: 22203994
18. p67(phox) plays a critical role in supporting reactive oxygen species production at the level of individual phagosomes. PMID: 21954286
19. Association analysis identified five SLE susceptibility genes reaching genome-wide levels of significance: NCF2, IKZF1, IRF8, IFIH1, and TYK2 PMID: 22046141
20. Genetic variation in the NCF2 gene was found to be associated with SLE in both US and European populations. PMID: 20842512
21. High NCF2 expression in the cytoplasm is associated with uterine cervix carcinogenesis. PMID: 21119665
22. The extended activation domain of p67(phox) (amino acids 190-210) containing the D(Y/F)LGK motif plays a crucial role in oxidase activation, likely by interacting with gp91(phox). PMID: 20679349
23. Mutations in CYBB, NCF1, CYBA, or NCF2 may contribute to the development of chronic granulomatous disease. PMID: 18546332
24. There is an increased expression of NADPH oxidase p47(-PHOX) and p67(-PHOX) factor in patients with idiopathic pulmonary fibrosis. PMID: 17651608
25. All mutations and some polymorphisms identified in the NCF2 gene in the autosomal forms of chronic granulomatous disease are listed. Review. PMID: 20167518
26. Our research demonstrates that p67(phox) adopts an elongated conformation when it exists not only as a monomer but also as the heterotrimer. PMID: 20375610
27. These findings identify the activation of PKC delta and NADPH oxidase as crucial steps in retinoic acid-induced neuroblastoma cell differentiation. PMID: 20074641
28. Alu-induced deletion of the TPR4 domain of p67-phox leads to a loss of function and accelerated degradation of the protein. PMID: 19953534
29. In a cell-free system, covalent binding between C-terminal-truncated p67phox and rac in the correct fusion order results in a more stable complex compared to the individual components. This complex significantly influences the duration of fusion-produced oxidase activation. PMID: 11705402
30. A detailed study of the protein-protein interactions that occur in the p40-p47-p67(phox) complex of the resting oxidase. PMID: 11796733
31. p22(phox), gp91(phox), p47(phox), p67(phox), and p40(phox) existed as a functional complex in the cytoskeletal fraction. PMID: 11893732
32. Val204 in p67(phox), previously found to be necessary for NADPH oxidase activity in cell-free conditions, was determined to be essential for superoxide production by intact COS-phox cells. PMID: 11929750
33. The effect of cPLA2 on its translocation. PMID: 12101222
34. NAD(P)H oxidase subunits p47(phox) and p67(phox) are expressed in platelets. NAD(P)H oxidase-dependent platelet superoxide anion release enhances platelet recruitment. PMID: 12130503
35. p67phox and p47phox play roles in regulating a conformational change in cytochrome b558, initiating electron transfer in NADPH oxidase activation. PMID: 12719414
36. NOXO1, p47phox, and p67phox regulate Nox3. PMID: 15181005
37. NAD(P)H oxidase activity is associated with increased protein levels of p22phox, p47phox, and p67phox, and increased p22phox and nox2 (gp91phox) mRNA expression. PMID: 15256399
38. Increased expression and activity of NAD(P)H oxidase subunits and xanthine oxidase, partly mediated through angiotensin II and PKC-dependent pathways, are significant mechanisms underlying increased oxidative stress in human coronary artery disease. PMID: 16293794
39. Our research reveals that the p47(phox)-p67(phox) interaction is disrupted not only by deletion of the PRR but also by substitution for basic residues in the extra-PRR (K383E/K385E). PMID: 16297854
40. Expression of p67(phox) is regulated through mechanisms that include modulation of transcription and translation. PMID: 16310324
41. These results indicate that Hcy (homocysteine)-stimulated superoxide anion production in monocytes is regulated through PKC-dependent phosphorylation of p47phox and p67phox subunits of NADPH oxidase. PMID: 16626305
42. NADPH oxidase assembly from p67phox was studied at the single-cell level. PMID: 16987007
43. Chemoattractant-stimulated superoxide production can be amplified by a positive feedback loop in which p67(phox) targets Vav1-mediated Rac activation. PMID: 17060455
44. These data clearly identify PLAGL2 as a novel regulator of NCF2/p67phox gene expression, as well as NADPH oxidase activity, contributing to a deeper understanding of the transcriptional regulation of NCF2. PMID: 17462995
45. A novel single nucleotide polymorphism in the promoter region. PMID: 17712795
46. This single nucleotide polymorphism leads to alternative splicing without altering gene expression or respiratory burst activity. PMID: 17910042
47. p40(phox) translocates p67(phox) to the region of the cytochrome and subsequently switches the oxidase to an activated state dependent upon PtdIns(3)P and SH3 domain engagement. PMID: 18029359
48. As(2)O(3) induced phosphorylation and membrane translocation of the NADPH oxidase subunit p47(phox), and it also increased translocation of Rac1 and p67(phox). PMID: 18424721
49. Autosomal recessive CGD due to NCF-2 gene mutations, and a novel homozygous and hypomorphic NCF-2 gene mutation was discovered. PMID: 18625437
50. p67(phox)-SH3(N) specifically functions in gp91(phox)/Nox2 activation, likely by facilitating oxidase assembly. PMID: 19116138

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HGNC: 7661

OMIM: 233710

KEGG: hsa:4688

STRING: 9606.ENSP00000356505

UniGene: Hs.587558