NDR1 Antibody
Description
Introduction to NDR1 Antibody
NDR1 (Nuclear Dbf2-related kinase 1) is a protein kinase involved in immune regulation, cell cycle control, and stress responses. NDR1 antibodies are specialized reagents designed to detect and study the expression, localization, and functional roles of the NDR1 protein across various biological systems. These antibodies are critical tools in molecular biology, enabling researchers to investigate NDR1’s involvement in diseases such as cancer, autoimmune disorders, and infectious diseases.
Types of NDR1 Antibodies and Their Characteristics
Key Features:
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A-8 and YJ-7: Detect nuclear-localized NDR1, critical for cell cycle regulation and immune signaling.
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Polyclonal Antibody: Used in plant studies to investigate NDR1’s role in disease resistance and cell wall integrity .
Immune Response Regulation
NDR1 antibodies have been pivotal in elucidating NDR1’s role in innate and adaptive immunity:
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Viral/Bacterial Infections: NDR1 deficiency reduces type I interferon (IFN) and pro-inflammatory cytokine production (e.g., IL-6, TNF-α) in macrophages, as shown using A-8 antibody in VSV-infected RAW264.7 cells .
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IL-17 Signaling: NDR1 promotes IL-17-induced inflammation in astrocytes and peritoneal mesothelial cells, confirmed via NDR1 knockdown and antibody-based validation .
Cancer and Disease Pathogenesis
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Breast Cancer: NDR1 modulates NOTCH1 signaling by impairing Fbw7-mediated degradation, enhancing cancer stem cell survival. Antibody-based studies in MCF-7 and SUM149 cells revealed NDR1’s role in promoting BCSC proliferation .
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Autoimmune Diseases: NDR1 deficiency in non-hematopoietic cells reduces the severity of experimental autoimmune encephalomyelitis (EAE) and TNBS-induced colitis, as demonstrated in Ndr1-KO mice .
Neurobiology and Cellular Stress
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Neuronal Function: Immunostaining with A-8 antibody in hippocampal neurons highlighted NDR1’s cytoplasmic localization and potential role in dendritic spine dynamics .
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DNA Repair: NDR1 interacts with MGMT to stabilize this DNA repair enzyme, though antibody-based studies in this context remain limited .
Table 1: NDR1 Antibody Applications in Disease Models
Table 2: NDR1 Antibody Performance in Detection Assays
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- NDR1 exhibits a broad role in mediating primary cellular functions in Arabidopsis, including maintaining the integrity of the cell wall-plasma membrane connection. It also acts as a key signaling component in defense responses during pathogen infection. PMID: 21398259
- The induction of defense responses and disease resistance to X. campestris pv. campestris strain 8004 requires NDR1, RAR1, and SGT1b, suggesting that effector-triggered immunity plays a significant role in resistance to this strain. PMID: 21029323
- Sequence analysis and mass spectrometry indicate that NDR1 is localized to the plasma membrane via a C-terminal glycosylphosphatidyl-inositol (GPI) anchor. PMID: 15447649
- Research has shown that the interaction between RIN4 and NDR1 occurs on the cytoplasmically localized N-terminal portion of NDR1, and this interaction is essential for the activation of resistance signaling following infection by P. syringae. PMID: 17012600
- Mutations in NDR1 abolish the enhanced resistance of dnd mutants against Pseudomonas syringae pv. tomato and Hyaloperonospora parasitica but not Botrytis cinerea. PMID: 18785824
