At5g52840 Antibody

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Description

Definition and Target

The At5g52840 Antibody is a monoclonal or polyclonal reagent designed to detect the protein encoded by the At5g52840 locus in Arabidopsis thaliana. This gene is annotated as a DNA-directed RNA polymerase subunit (UniProt ID: Q9FLX7) involved in transcriptional regulation .

Role in Stress Response

A 2017 study identified At5g52840 as significantly upregulated (10.3-fold, log₂ 3.4) in Arabidopsis leaves exposed to ochratoxin A (OTA), a mycotoxin triggering programmed cell death (PCD) . This induction suggests its involvement in stress-related RNA metabolism.

Gene IDDescriptionFold Change (log₂)Function
At5g52840DNA-directed RNA polymerase3.4RNA synthesis under stress

Associated Pathways

  • Linked to RNA polymerase activity during PCD .

  • Potential role in transcriptional regulation during oxidative stress .

Applications in Research

  • Stress Response Studies: Detecting At5g52840 expression under abiotic/biotic stress conditions .

  • Transcriptional Regulation: Investigating RNA polymerase complexes in plant immunity .

  • Protein Localization: Subcellular tracking via immunohistochemistry .

Limitations and Future Directions

  • Functional Data Gap: The exact molecular role of At5g52840 remains uncharacterized beyond transcriptional associations.

  • Validation Needs: Independent studies confirming antibody specificity are lacking .

Product Specs

Buffer
**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
At5g52840 antibody; MXC20.6 antibody; Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 antibody; mitochondrial antibody
Target Names
At5g52840
Uniprot No.

Target Background

Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), believed to be non-catalytic. Complex I facilitates electron transfer from NADH to the respiratory chain. The immediate electron acceptor for this enzyme is thought to be ubiquinone.
Database Links

KEGG: ath:AT5G52840

STRING: 3702.AT5G52840.1

UniGene: At.29591

Protein Families
Complex I NDUFA5 subunit family
Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

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