NDUFB2 Antibody

Introduction

The NDUFB2 antibody targets the NDUFB2 protein, a subunit of Complex I (NADH dehydrogenase [ubiquinone] 1 beta subcomplex, 2) in the mitochondrial electron transport chain. This antibody is widely used in research to study mitochondrial function, oxidative phosphorylation, and diseases associated with Complex I dysfunction.

Structure and Function of NDUFB2

• Gene Location: The NDUFB2 gene is located on chromosome 7q34, spanning 9,966 base pairs and comprising 4 exons .

• Protein Characteristics: The encoded protein is a 12 kDa mitochondrial accessory subunit with a hydrophobic transmembrane domain and a hydrophilic domain interacting with Complex I’s peripheral arm .

• Role in Complex I: NDUFB2 stabilizes the complex but does not directly participate in catalysis. It facilitates electron transfer from NADH to ubiquinone, a critical step in ATP synthesis .

Applications of NDUFB2 Antibody

The antibody is employed in:

• Western Blot (WB): Detects a 12 kDa band in human, mouse, and rat tissues (e.g., skeletal muscle, brain) .

• Immunohistochemistry (IHC): Localizes NDUFB2 in mitochondrial-rich tissues (e.g., heart, liver) .

• Immunofluorescence (IF): Visualizes mitochondrial localization in cell lines like C2C12 .

Research Findings

• Venous Thromboembolism (VTE): High NDUFB2 expression correlates with VTE in cirrhotic patients, suggesting its role in mitochondrial dysfunction and oxidative stress .

• Cancer Prognosis: Elevated NDUFB2 levels are linked to poor survival in glioblastoma and gliomas .

• Mitochondrial Disorders: Mutations in NDUFB2 are implicated in Complex I deficiency, a cause of neurodegenerative diseases .