NDUFV3 Human
Description
Gene and Protein Overview
The protein sequence (35–108 aa for NDUFV3-S) includes a disordered N-terminal region in NDUFV3-L (423 additional residues), which may mediate interactions with other proteins or signaling molecules .
Primary Responsibilities
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Accessory Subunit: Facilitates Complex I assembly and stability but is not directly involved in catalysis .
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Terminal Assembly: May act as a "terminally assembled" subunit, ensuring proper structural integrity during final stages of complex formation .
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Isoform-Specific Functions:
Key Binding Partners
These interactions are critical for maintaining the structural and functional integrity of Complex I .
Knockout Studies
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HEK293T Cell Models:
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Y. lipolytica Models:
Expression in Cancer
*Data from immunohistochemical staining (Human Protein Atlas) .
Survival Correlations
No significant association between NDUFV3 expression and survival outcomes in major cancers (e.g., colorectal, breast) .
Recombinant Protein
Product Specs
Product Science Overview
NDUFV3 is a 108 amino acid protein that is believed not to be involved in catalysis . Instead, it plays a structural role within the complex. Mitochondrial complex I functions by oxidizing NADH produced in the Krebs cycle, utilizing the two electrons to reduce ubiquinone to ubiquinol. This initiates the passage of electrons to successive complexes, ultimately leading to the reduction of oxygen to water .
Defects in any of the subunits of mitochondrial complex I, including NDUFV3, can lead to various myopathies and neuropathies . This makes it of considerable clinical interest. Although NDUFV3 itself is not directly involved in catalysis, its proper function is crucial for the stability and efficiency of the entire complex .
The recombinant form of NDUFV3 is typically expressed in Escherichia coli and purified using affinity chromatography techniques. The protein is often tagged with a His-ABP tag to facilitate purification . The purified protein is stored in a buffer containing 1M urea/PBS at pH 7.4 and is kept at -20°C to avoid freeze/thaw cycles .
NDUFV3 is used in various research applications, including studies on mitochondrial function and the electron transport chain. It serves as a control fragment in blocking experiments with corresponding antibodies . Researchers also use it to investigate the structural and functional aspects of mitochondrial complex I and its role in cellular respiration .
