NEAP2 Antibody
Description
Potential Misinterpretations
The term "NEAP2" may represent a typographical error or nomenclature confusion. The following antibodies with similar naming patterns appear in scientific literature:
Note: MAP2 is extensively documented in the provided sources (e.g., NBP2-50032, AB5622) and serves as a validated neuronal marker.
Detailed Analysis of MAP2 Antibody (Closest Match)
MAP2 (Microtubule-Associated Protein 2) antibodies are widely used in neuroscience research. Key characteristics include:
Key Features of MAP2 Antibodies
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Target: MAP2A/B (280 kDa) and MAP2C/D (70 kDa) isoforms.
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Applications:
Research Applications
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Neuronal Differentiation: Used to validate induced pluripotent stem cell-derived neurons ( ).
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Synaptic Plasticity: Correlates with improved hippocampal information processing ( ).
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Pathology: Detects axonal integrity in prion disease models ( ).
Antibody Validation Data (MAP2 Example)
Data from source (NBP2-50032 monoclonal antibody):
| Parameter | Details |
|---|---|
| Host Species | Mouse |
| Clone | 2C4 |
| Applications | WB, IHC, ICC/IF |
| Specificity | All MAP2 isoforms |
| Immunogen | Full-length human MAP2D |
Western Blot Performance
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SH-SY5Y Cells: Strong bands at 280 kDa (MAP2A/B) and 70 kDa (MAP2C/D).
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Specificity: No cross-reactivity in HEK293 or NIH/3T3 cells ( ).
Recommendations for Clarification
If "NEAP2" refers to a novel or proprietary antibody, the following steps are advised:
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Verify the correct nomenclature with the originating institution or publication.
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Cross-reference with repositories like:
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UniProt (https://www.uniprot.org)
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NCBI Protein (https://www.ncbi.nlm.nih.gov/protein)
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Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- AtNEAPs are proposed as inner nuclear membrane-anchored coiled-coil proteins that play a role in maintaining nuclear morphology and chromatin structure. [NEAP2] PMID: 27630107
KEGG: ath:AT5G26770
UniGene: At.30845
Q&A
FAQs for NEAP2 Antibody Research
Advanced Research Questions
How can experimental design optimize NEAP2 antibody validation for high-throughput screening?
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Design of Experiments (DOE): Use multifactorial testing to evaluate critical parameters (e.g., antibody dilution, incubation time) with minimal runs .
Example DOE Table:
How to resolve contradictions in NEAP2 expression data across published studies?
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Meta-analysis: Compare datasets using standardized normalization methods (e.g., housekeeping protein ratios) .
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Technical audit: Re-evaluate antibody clones, fixation protocols, and scoring criteria across studies .
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Orthogonal validation: Confirm findings with RNA-seq or CRISPR-Cas9 knockout models .
What strategies enhance epitope mapping for NEAP2 antibodies in structural studies?
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Phage display libraries: Screen random peptide libraries to identify binding motifs .
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X-ray crystallography: Resolve antibody-antigen complexes to define epitope-paratope interfaces .
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Alanine scanning mutagenesis: Systematically mutate NEAP2 residues to pinpoint critical binding regions .
