NECTIN4 Antibody, Biotin conjugated
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Description
Introduction
Nectin-4 (NECTIN4) is a transmembrane protein overexpressed in various cancers, including urothelial, breast, and ovarian carcinomas, and correlates with poor prognosis . The NECTIN4 Antibody, Biotin conjugated, is a rabbit polyclonal antibody developed for research applications, leveraging biotin labeling for enhanced sensitivity in assays requiring avidin-biotin detection systems .
Applications
The antibody is primarily validated for ELISA to detect Nectin-4 in human samples . Its biotin conjugation enables compatibility with streptavidin-based detection systems, enhancing assay sensitivity. Potential applications include:
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Biomarker analysis: Quantifying Nectin-4 expression in tumor tissues or sera to assess cancer progression.
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Immunohistochemistry (IHC): Localizing Nectin-4 in tissue sections (requires appropriate secondary reagents).
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Western blot: Detecting Nectin-4 in lysates, though optimization may be needed.
Role of Nectin-4 in Cancer
Nectin-4 is a validated target for antibody-drug conjugates (ADCs) like enfortumab vedotin (EV), approved for urothelial cancer . Overexpression of Nectin-4 correlates with tumor aggressiveness and poor survival , making it a critical biomarker for therapeutic strategies.
Utility in ADC Development
The antibody’s specificity for Nectin-4 aligns with research on next-generation ADCs, such as 9MW2821, which demonstrates superior efficacy and safety compared to EV in preclinical models . Its biotin labeling facilitates integration into assays for validating ADC binding or tumor targeting.
CAR-T Cell Therapies
Nectin-4-targeted CAR-T cells, such as Nectin4-7.19 CAR-T, show promise in eradicating metastatic tumors in preclinical models . The antibody supports studies on CAR-T cell specificity and tumor antigen recognition.
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
We typically dispatch products within 1-3 business days after receiving your order. Delivery time may vary based on the purchase method or location. Please consult your local distributor for specific delivery timelines.
Synonyms
DKFZp686K05193 antibody; EDSS1 antibody; Ig superfamily receptor LNIR antibody; Nectin 4 antibody; Nectin-4 antibody; poliovirus receptor-related 4 antibody; Processed poliovirus receptor-related protein 4 antibody; PRR4 antibody; pvrl4 antibody; PVRL4_HUMAN antibody
Target Names
Uniprot No.
Target Background
Function
NECTIN4, also known as PVRL4, is a cell adhesion molecule that plays a role in cell adhesion through trans-homophilic and -heterophilic interactions. It specifically interacts with NECTIN1 and does not serve as a receptor for alpha-herpesvirus entry into cells. Notably, it functions as a receptor for measles virus entry into cells.
Gene References Into Functions
- Research suggests that Nectin-4 is a significant prognostic factor and a potential therapeutic target in luminal B (HER2 negative) breast cancer. PMID: 28778498
- Nectin-4 promotes cell-cell adhesion/aggregation, migration, and proliferation of ovarian tumor cells. PMID: 28038455
- Nectin-4 has been identified as a marker for breast cancer stem cells (BCSCs) and a key player in breast cancer metastasis. PMID: 28600142
- Studies have shown that PVRL4 is post-transcriptionally regulated by miR-128 and miR-31. PMID: 27507538
- Elevated Nectin-4 expression is associated with the development of neoplasms. PMID: 27013195
- Research indicates that Measles virus enters MCF7, DLD-1, and HTB-20 cancer cells through a PVRL4-mediated macropinocytosis pathway, involving specific cellular GTPases and kinases. PMID: 28250131
- Nectin-4 acts as a stimulatory co-receptor for the prolactin receptor, influencing the feedback inhibition of SOCS1 in the JAK2-STAT5a signaling pathway. PMID: 28258213
- Nectin-4 emerges as a promising prognostic biomarker and a specific therapeutic target for Triple-negative breast cancers. PMID: 27998973
- ADAM17 and ADAM10 cleave Nectin-4, leading to the release of soluble Nectin-4 (sN4). PMID: 28232483
- Studies suggest that Nectin-4 may serve as a therapeutic target for systemic lupus erythematosus by influencing cell apoptosis. PMID: 26617807
- Nectin-4 is critical for gallbladder cancer cell progression by activating the PI3K/AKT pathway and Rac1. PMID: 26949052
- Nectin-4 expression is elevated in endometriotic lesions of patients with ovarian endometriosis and peritoneal endometriosis, particularly in the endometrium of patients with endometriosis. PMID: 27328518
- Transformation of breast cancer cells is dependent on PVRL4. PMID: 23682311
- Nectin-4 is a significant prognostic predictor and may play a critical role in pancreatic cancer, potentially serving as a novel therapeutic target for this disease. PMID: 25888293
- In airway epithelial cells, measles virus spread relies on the Nectin-4/afadin complex and involves cytoplasm transfer between columnar cells. PMID: 25926640
- Clinical investigations of the EDSS1, a genetic disorder, have identified a novel homozygous nonsense mutation (181C>T, p.Asp61*) in the PVRL4 gene. PMID: 25529316
- Ovarian tissue expression and serum Nectin-4 appear to be potential markers for ovarian cancer. PMID: 25019423
- Research has shown that the same key residues in the BC and FG loops of Nectin-4 govern binding to the measles virus attachment protein hemagglutinin (H) and cell entry, Nectin-4 homodimerization, and heterodimerization with Nectin-1. PMID: 25275122
- Data highlights a synergistic action of Nectin-1 and -4 in the early stages of adherens junction (AJ) formation, and this interaction influences the Rac1 signaling pathway. PMID: 24577405
- Eliminating Nectin4 binding compromises CD46 usage by a hybrid vesicular stomatitis virus/measles virus. PMID: 24335299
- While Nectin-4 and CD46 interact functionally with the measles virus H protein beta4-beta5 hydrophobic groove, SLAM merely covers it. PMID: 23760251
- Research indicates that Nectin-4 expression is up-regulated in the eutopic endometrium of patients with endometriosis compared to control subjects. PMID: 22926846
- Studies have demonstrated that human Nectin4 is fully functional as a receptor for canine distemper virus. PMID: 23174504
- The structure of the membrane-distal domain of Nectin-4 in complex with measles virus hemagglutinin (MV-H) reveals that Nectin-4 binds the MV-H beta4-beta5 groove exclusively via its N-terminal IgV domain. The contact interface is dominated by hydrophobic interactions. PMID: 23202587
- Crystals of Nectin-4 diffracted to 1.8 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 33.1, b = 51.7, c = 56.9 Å, beta = 94.7 degrees. PMID: 22869128
- Measles virus utilizes Nectin-4 to emerge in the airways. PMID: 22048310
- Measles virus infection reduces PVRL4 surface expression in MCF7 cells, a characteristic of receptor-associated viral infections. PMID: 21901103
- Research suggests that increased expression of Nectin-4 may indicate a worse prognosis in breast cancer patients. PMID: 21526486
- Sequence analysis revealed a homozygous missense mutation (c.635C>G; p.Pro212Arg) in the PVRL4 gene, causing EDSS1. PMID: 21346770
- Ectoderma dysplasia-syndactyly syndrome (EDSS1) is the second known "nectinopathy", caused by mutations in a nectin adhesion molecule. PMID: 21333831
- Nectin-4 may serve as a potential biomarker to differentiate benign gynecologic diseases from ovarian cancer, particularly when used in combination with CA125. PMID: 20959669
- EDSS1 is the second known "nectinopathy", caused by mutations in a nectin adhesion molecule. PMID: 20691405
- The active form of TACE (tumor necrosis factor-alpha converting enzyme) is overexpressed in breast tumors, indicating a potential role of TACE in Nectin-4 shedding both in vitro and in vivo. PMID: 15784625
- Nectin-4 was not detected in normal breast epithelium, but it was expressed in ductal breast carcinoma. PMID: 17474988
- Nectin-4 might play a significant role in lung carcinogenesis and could serve as a novel serum and tissue biomarker, as well as a therapeutic target. PMID: 19679554
Database Links
Involvement In Disease
Ectodermal dysplasia-syndactyly syndrome 1 (EDSS1)
Protein Families
Nectin family
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction. Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254).; [Processed poliovirus receptor-related protein 4]: Secreted. Note=The secreted form is found in breast tumor patients (PubMed:15784625).
Tissue Specificity
Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
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