NFIB Antibody
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Description
Understanding NFIB Antibody
The NFIB Antibody is a polyclonal antibody developed to target the Nuclear Factor I/B (NFIB) protein, a transcription factor involved in DNA binding and gene regulation. This antibody is widely used in molecular biology research to study NFIB's role in cellular processes, including cancer progression, stem cell maintenance, and developmental biology.
Validated Applications
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Western Blotting: Detects NFIB in HUVEC, HeLa, HepG2, and NIH/3T3 cells .
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IHC: Effective in human skin cancer tissues with antigen retrieval using TE buffer pH 9.0 .
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Flow Cytometry: Demonstrated in HeLa cells for intracellular staining .
Research Applications of NFIB Antibody
NFIB has emerged as a critical target in oncology due to its oncogenic role in multiple cancers. Below are key findings from recent studies:
Breast Cancer
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Triple-Negative Breast Cancer (TNBC): NFIB mRNA is highly expressed in TNBC tissues, correlating with poor prognosis .
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ER-Negative Tumors: NFIB protein upregulation associates with high nuclear grade and HER2 overexpression .
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Therapeutic Targeting: siRNA-mediated NFIB knockdown reduces proliferation and induces apoptosis in HCC1954 cells .
Colorectal Cancer (CRC)
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Oncogenic Role: NFIB promotes CRC cell proliferation, migration, and resistance to 5-fluorouracil (5-FU) .
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Mechanism: Activates the Akt signaling pathway, enhancing tumor growth in xenograft models .
Other Cancers
Product Specs
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. Store at -20°C. Avoid repeated freeze-thaw cycles.
Lead Time
Typically, we can ship your orders within 1-3 business days of receipt. Delivery times may vary depending on the purchase method and location. Please consult your local distributor for specific delivery details.
Synonyms
CCAAT Box Binding Transcription Factor antibody; CCAAT-box-binding transcription factor antibody; CTF antibody; HMGIC/NFIB antibody; NF-I/B antibody; NF1-B antibody; NF1B antibody; NF1B2 antibody; NFI-B antibody; NFI-RED antibody; Nfib antibody; NFIB_HUMAN antibody; NFIB2 antibody; NFIB3 antibody; Nuclear factor 1 B-type antibody; Nuclear factor 1/B antibody; Nuclear Factor 1B antibody; Nuclear Factor I B antibody; Nuclear factor I/B antibody; TGGCA Binding Protein antibody; TGGCA-binding protein antibody; TRANSCRIPTION FACTOR NFIB antibody
Target Names
NFIB
Uniprot No.
Target Background
Function
NFIB (Nuclear Factor I/B) is a transcription activator that plays a crucial role in proper brain development. It binds to the palindromic sequence 5'-TTGGCNNNNNGCCAA-3', which is present in viral and cellular promoters as well as the origin of replication of adenovirus type 2. This protein is capable of activating both transcription and replication.
Gene References Into Functions
- High NFIB expression has been linked to cell growth, aggressiveness, and metastasis in gastric cancer. PMID: 30015981
- Research has shown that miR-30d suppresses cell migration and invasion in non-small cell lung cancer (NSCLC) by directly targeting NFIB. PMID: 28861760
- Studies have highlighted the complex role of NFIB in tumor development and progression, where it can both inhibit and promote tumor growth depending on the specific cancer type and tissue. This underscores its diverse and tissue-specific functions. [review] PMID: 28596133
- Research indicates that NFIB is associated with improved survival in patients with glioblastoma. PMID: 27083054
- Elevated NFIB levels have been observed to correlate with the expansive growth of poorly differentiated and invasive tumor cells that lack E-cadherin (CDH1) expression. PMID: 27373156
- Low NFIB expression is associated with more aggressive subtypes of lung adenocarcinoma and poorer survival in patients. PMID: 27357447
- Evidence suggests that NFIB increases EZH2 protein expression downstream of BRN2 protein, leading to decreased MITF protein levels. PMID: 28119061
- In benign prostatic hyperplasia, loss of luminal NFIB expression correlates with more severe disease. Studies in androgen-dependent LNCaP prostate cancer cells have demonstrated that a significant portion of NFIB binding sites are associated with androgen receptor (AR) and FOXA1 binding sites. PMID: 26677878
- Studies suggest that genetic variations in the NFIB gene are associated with osteosarcoma metastasis, indicating its potential role in metastasis susceptibility. PMID: 26084801
- Further research has uncovered a regulatory loop between NFIB and miR-365 that plays a role in the development of cutaneous squamous cell carcinoma. PMID: 24949940
- Combined overexpression of NFIB, RANK, and RANKL has been linked to a significantly increased risk of metastasis in cancer. PMID: 25764026
- Increased NFIB expression is associated with pilocytic astrocytoma. PMID: 23161775
- Research has demonstrated that the autism spectrum disorder-associated A-C intronic haplotype of the ENGRAILED2 gene acts as a transcriptional activator, with both CUX1 and NFIB mediating this activity. PMID: 22180456
- Immunohistochemistry analysis of invasive breast cancer patients revealed a significant association between NFIB expression and nuclear grade, estrogen receptor, and HER2 expression status. PMID: 21925980
- Studies have shown that microRNAs (miRs-372/373) can promote HBV expression through a pathway involving NFIB. PMID: 21608007
- Functional studies suggest that NFIB plays a role in regulating cell viability and proliferation during tumor transformation. PMID: 21764851
- Research indicates that NFIB is a potential biomarker for oxaliplatin resistance in human cancers. PMID: 21087353
- The MYB-NFIB fusion is characteristic of a subset of adenoid cystic carcinomas (ACCs) and contributes to MYB overexpression. PMID: 20702610
- Expression of the NFI-B2 isoform has been shown to impair CD4 transcription in CD4-positive primary T-lymphocytes and cell lines during HIV-1 infection. PMID: 12639247
- The C-terminal domain of the nuclear factor I-B2 isoform is glycosylated and transactivates the WAP gene in tumor cells. PMID: 17511965
- Rearrangement of NFIB may be associated with deep-seated lipomas, such as retroperitoneal or gastro-intestinal lipomas. PMID: 18663748
- Knockdown of NFIB expression using siRNA has been shown to decrease IGFBP5 expression, while knockdown of NFIC expression increased IGFBP5 expression. PMID: 18809517
- Lipomas containing NFIB rearrangements may exhibit specific clinicohistologic features, including large size, deep location, infiltration of surrounding muscles, or early onset. PMID: 19837271
Database Links
Protein Families
CTF/NF-I family
Subcellular Location
Nucleus.
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