NID2 Antibody
Description
NID2 Antibody Overview
NID2 antibodies target the Nidogen-2 glycoprotein, a 200 kDa secreted protein encoded by the NID2 gene (Accession # Q14112) . Key features include:
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Structure: Mature NID2 contains three globular domains (G1–G3), a mucin-like linker region, and motifs for binding laminins, collagen IV, and integrins .
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Function: Facilitates BM integrity by linking laminin and collagen networks, influencing tissue biomechanics and cellular signaling .
Applications of NID2 Antibodies
NID2 antibodies are used across diverse experimental contexts:
Role in Cancer Progression
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Pancreatic Cancer: High stromal NID2 expression correlates with poor survival and increased metastasis. CRISPRi-mediated NID2 knockdown in cancer-associated fibroblasts (CAFs) reduced collagen cross-linking, matrix stiffness, and cancer cell invasion in 3D models .
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Therapeutic Potential: NID2-depleted tumors showed enhanced chemotherapy (gemcitabine/Abraxane) efficacy, reduced liver metastasis, and improved vascular patency in murine models .
Biomarker Utility
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NID2 promoter hypermethylation and subsequent protein loss are linked to gastric and lung carcinoma metastasis .
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In pancreatic ductal adenocarcinoma (PDAC), NID2 is predominantly expressed by CAFs and stellate cells, making it a stromal biomarker for aggressive subtypes .
Mechanistic Insights
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Matrix Interaction: NID2 binds laminin via its G3 domain and collagen IV via G1, stabilizing BMs. Loss disrupts BM integrity, promoting epithelial cell invasion .
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Signaling Pathways: NID2 depletion alters LOX-mediated collagen cross-linking and reduces SHG signal intensity, indicative of fibrillar collagen disorganization .
Clinical and Preclinical Implications
Product Specs
Target Background
Gene References and Functional Implications:
- Elevated NID2 methylation has been associated with non-small cell lung cancer. PMID: 29970687
- Serum NID2 levels are significantly elevated in patients with esophageal squamous cell carcinoma (ESCC). Higher serum NID2 levels (≥ 32.6 μg/L) are strongly correlated with poor survival outcomes compared to those with very low levels (< 20.0 μg/L). PMID: 29278876
- Adding a TWIST1/NID2-based DNA methylation assay to urine cytology analysis enhances diagnostic accuracy, particularly for equivocal cytology cases. PMID: 28106542
- Promoter methylation of NID2 is significantly higher in nasopharyngeal carcinoma and esophageal squamous cell carcinoma cells. NID2 suppresses the EGFR/Akt and integrin/FAK/PLCgamma metastasis-related pathways. PMID: 27793011
- Knockdown of nidogen-2 reduces SOX9 expression while upregulating RUNX2 expression. PMID: 26683663
- The cleavage of nidogen-1 and -2 is partially inhibited in human tumor samples. PMID: 23681936
- Combined methylation analysis of Twist homolog 1 and nidogen-2 genes provides a simple, non-invasive, sensitive, and specific method for detecting cancer cells in urine. PMID: 23682613
- Decreased nidogen-2 expression is associated with hepatocellular carcinoma. PMID: 21815147
- NID2 promoter hypermethylation is associated with oral cavity squamous cell carcinoma. PMID: 21558411
- Nidogen-2 is a novel biomarker for ovarian cancer that correlates closely with CA125. PMID: 19883638
- The short arm region of the laminin-5 gamma2 chain: structure, mechanism of processing, and binding to heparin, nidogen-1, fibulin-1, and fibulin-2. PMID: 11733994
- Nidogen-2 has been identified as a ubiquitous component of basement membrane zones beneath developing epithelia in most major organ systems, potentially participating in cell biological functions during human development. PMID: 12005023
- The nidogen-2 compensation hypothesis has been proposed to explain the limited phenotype observed following the elimination of the nidogen-1 gene. PMID: 12475645
- Nidogen-2 has been found to be functionally equivalent to nidogen-1 in skin organotypic coculture, and both can promote the development of a functional basement membrane zone. PMID: 17008882
- CpG islands in both NID1 and NID2 genes are aberrantly methylated in human cancer samples and cancer cell lines. PMID: 17328794
