NIK1 Antibody
Description
Definition and Target Specification
NIK1 antibodies are immunoglobulin-based reagents designed to bind specifically to the NIK protein. In mammalian systems, NIK (MAP3K14) regulates immune responses and cell survival through NF-κB activation . In plants, NIK1 is a receptor-like kinase involved in antiviral defense mechanisms . This article focuses on the mammalian NIK antibody due to its commercial availability and well-documented applications.
Mechanistic Insights in Mammalian Systems
NIK1 antibodies enable the study of NF-κB signaling pathways, which are critical in inflammation, lymphoid organ development, and B-cell maturation . For example:
-
Detects endogenous NIK expression levels in immune cell lysates .
-
Facilitates analysis of NIK phosphorylation states under cytokine stimulation .
Plant NIK1 Context
Although not directly related to the commercial #4994 antibody, plant NIK1 studies reveal its role in antiviral immunity:
-
Antiviral Action: NIK1 phosphorylates ribosomal protein RPL10, suppressing viral mRNA translation .
-
Bacterial Immunity Modulation: NIK1 inhibits FLS2/BAK1 complex formation, reducing antibacterial responses .
-
Genetic Evidence: nik1-1 mutants exhibit enhanced resistance to Pseudomonas syringae but increased susceptibility to RNA/DNA viruses .
Technical Considerations
-
Cross-Reactivity: The #4994 antibody does not recognize plant NIK1 homologs .
-
Validation: Ensure antibody specificity via knockout controls in WB experiments .
-
Storage: Follow manufacturer guidelines (-20°C in aliquots) to preserve activity .
Comparative Analysis of NIK1 Functions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
This antibody targets NIK1, a histidine kinase involved in a two-component signaling pathway regulating cell wall mannan biosynthesis. It is essential for hyphal formation and virulence. NIK1 plays a role in fungicide sensitivity, likely through the positive regulation of the HOG1-signaling pathway. It is presumed to mediate phosphotransfer to the HOG1 MAP kinase pathway during oxidative and potentially osmotic stress.
KEGG: cal:CAALFM_C702800WA
