NIP2 Antibody

Introduction to NIP2 Antibody

NIP2 (Nek2-Interacting Protein 2), also known as centrobin, is a centrosomal protein critical for microtubule organization, spindle assembly, and centriole duplication . Antibodies targeting NIP2 are essential tools for studying its roles in cell cycle regulation, mitotic processes, and pathologies such as cancer. Research on NIP2 antibodies has revealed insights into protein localization, phosphorylation dynamics, and functional interactions with kinases like Nek2 .

2.1. Functional Role in Microtubule Organization

• NIP2 stabilizes microtubule networks and supports microtubule nucleation activity. Knockdown experiments show impaired microtubule re-growth and spindle assembly defects .

• Mechanism: NIP2 phosphorylation by Nek2 regulates its mobilization to microtubules, enhancing structural stability .

2.2. Cell Cycle Regulation

• NIP2 levels remain stable throughout the cell cycle but decrease slightly during G2/M phase. Depletion leads to G2/M arrest, apoptosis, and abnormal nuclear morphology .

• Impact: Cells lacking NIP2 exhibit reduced viability (~50% survival at 96 hours post-transfection) .

2.3. Interaction with Nek2 Kinase

• NIP2 is a substrate of Nek2, with phosphorylation occurring at its N-terminal region (residues 1–193). This interaction modulates centrosomal NIP2 levels .

• Key Evidence:

  • Nek2 overexpression reduces centrosomal NIP2, while Nek2 suppression increases it .

  • In vivo labeling confirms NIP2 phosphorylation by active Nek2 .

NIP2 Antibody Applications

NIP2 antibodies are used in diverse experimental workflows:

5.1. Knockdown Effects (Source )

Clinical and Research Implications

NIP2 antibodies enable studies of centriole duplication errors linked to microcephaly and cancer . Ongoing research explores its role in BRCA2-mediated DNA repair pathways .