NMAT1 Antibody

Definition and Function of NMNAT1 Antibody

NMNAT1 antibodies are immunoglobulins designed to bind specifically to NMNAT1, a nuclear enzyme catalyzing the conversion of nicotinamide mononucleotide (NMN) to NAD⁺. This process is essential for maintaining nuclear NAD⁺ levels, which are critical for chromatin remodeling, DNA repair, and transcriptional regulation .

Key Applications:

• Western Blot (WB): Quantifying NMNAT1 protein levels in cell lysates.

• Immunohistochemistry (IHC): Localizing NMNAT1 in tissue sections (e.g., retinal, pancreatic, or kidney tissues) .

• Immunofluorescence (IF): Visualizing NMNAT1 in subcellular compartments .

• Immunoprecipitation (IP): Studying NMNAT1 interactions with other proteins .

Types of NMNAT1 Antibodies

NMNAT1 antibodies vary in host origin, epitope specificity, and reactivity. Below is a comparison of commercially available antibodies:

Notes:

• Polyclonal vs. Monoclonal: Polyclonal antibodies (e.g., Proteintech 11399-1-AP) target multiple epitopes, offering broader detection, while monoclonal antibodies (e.g., Santa Cruz B-7) provide higher specificity .

• Reactivity: Species-specific antibodies are critical for cross-species studies (e.g., mouse models for human diseases) .

Research Findings and Applications

NMNAT1 antibodies have been pivotal in elucidating NMNAT1’s roles in disease and metabolism:

Retinal Degeneration

• Mechanism: NMNAT1 knockout in murine retinas causes severe photoreceptor and inner retinal neuron loss due to disrupted NAD⁺ metabolism and activation of pyroptosis, necroptosis, and apoptosis .

• Antibody Use: Custom polyclonal antibodies confirmed drastic NMNAT1 protein reduction in knockout retinas (P0 mice) .

Amyloid Clearance and Neurodegeneration

• Mechanism: Overexpression of human NMNAT1 (hNmnat1) reduces amyloid plaques by enhancing autophagic clearance of amyloid-β (Aβ) in Drosophila models .

• Antibody Use: Antibodies detected NMNAT1 localization and autophagy markers (e.g., Atg8a) to validate its role in amyloid pathology .

Acute Myeloid Leukemia (AML)

• Mechanism: NMNAT1 sustains nuclear NAD⁺ levels, enabling AML progression and chemoresistance. Deletion of NMNAT1 reduces leukemic stem cell (LSC) survival and sensitizes cells to venetoclax .

• Antibody Use: Antibodies confirmed NMNAT1 knockdown in AML patient-derived xenograft (PDX) models .

Metabolic Regulation

• Mechanism: NMNAT1 regulates central carbon, purine, and amino acid metabolism. Its loss in retinas alters 39 metabolites, including NAD⁺ precursors and nucleotides .