NOLC1 Antibody
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Description
Applications in Research
The NOLC1 antibody has been employed in diverse studies, with a focus on ribosome biogenesis, cancer biology, and genome editing:
Role in Ribosome Biogenesis
NOLC1 is critical for nucleolar function, guiding site-specific 2'-O-methylation and pseudouridylation of pre-rRNAs . Overexpression disrupts rRNA processing, as shown by studies using this antibody to detect mislocalized nucleolar proteins .
Cancer Biology
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Breast Cancer: High NOLC1 expression correlates with poor prognosis in triple-negative breast cancer (TNBC), as demonstrated by immunohistochemical analysis .
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Nasopharyngeal Carcinoma: The antibody revealed that NOLC1 overexpression enhances tumorigenesis by activating MDM2 via TP53 .
Genome Editing
In a study on CRISPR-directed integrases, the antibody confirmed NOLC1’s interaction with β-arrestin and fibrillarin, highlighting its role in rRNA biogenesis during colorectal tumorigenesis .
Protocols and Usage Guidelines
Protocols for Western blotting, IHC, and IF/ICC are available from the manufacturer. Key considerations include:
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WB: Use 1:500–1:1000 dilution in TBST with 5% skim milk.
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IHC: Optimize antigen retrieval with citrate buffer (pH 6.0) for paraffin sections .
Publications and Validation
The antibody has been cited in studies on:
Product Specs
Buffer
The antibody is provided as a liquid solution in phosphate-buffered saline (PBS) containing 50% glycerol, 0.5% bovine serum albumin (BSA), and 0.02% sodium azide.
Form
Liquid
Lead Time
Generally, we can ship your orders within 1-3 business days after receiving them. Delivery times may vary depending on the purchase method and location. Please contact your local distributor for specific delivery time estimates.
Synonyms
140 kDa nucleolar phosphoprotein antibody; HCV NS5A trans regulated protein 13 antibody; HCV NS5A transactivated protein 13 antibody; HCV NS5A-transactivated protein 13 antibody; Hepatitis C virus NS5A transactivated protein 13 antibody; Hepatitis C virus NS5A-transactivated protein 13 antibody; KIAA0035 antibody; NOLC 1 antibody; NOLC1 antibody; NOLC1_HUMAN antibody; NOPP 130 antibody; NOPP 140 antibody; NOPP130 antibody; Nopp140 antibody; NS5ATP13 antibody; Nucleolar 130 kDa protein antibody; Nucleolar and coiled body phosphoprotein 1 antibody; Nucleolar and coiled body phosphprotein 1 antibody; Nucleolar and coiled-body phosphoprotein 1 antibody; Nucleolar phosphoprotein p130 antibody; Nucleolar protein p130 antibody; P130 antibody
Target Names
NOLC1
Uniprot No.
Target Background
Function
NOLC1, also known as Nopp140, is a nucleolar protein that plays a crucial role in regulating RNA polymerase I activity. It acts as a bridge between RNA polymerase I and enzymes involved in ribosomal processing and modification. NOLC1 is essential for neural crest specification. Upon monoubiquitination by the BCR(KBTBD8) complex, it associates with TCOF1, forming a platform that connects RNA polymerase I with enzymes responsible for ribosomal processing and modification. This leads to a shift in the translational program of differentiating cells, favoring neural crest specification. NOLC1 is also involved in nucleologenesis, likely through its role in maintaining the structural integrity of the fibrillar center and dense fibrillar component within the nucleolus. NOLC1 possesses intrinsic GTPase and ATPase activities.
Gene References Into Functions
- This study investigated the disordered nature of Nopp140 and the influence of CK2alpha on its structure using single-molecule fluorescence resonance energy transfer (smFRET) and electron paramagnetic resonance (EPR). PMID: 27297113
- D120 and R195 of H5N1 influenza virus NS1 were identified as crucial residues for binding to the host NOLC1 protein. PMID: 25645906
- Cytoplasmic delocalization of pRb2/p130 can lead to cell cycle deregulation. PMID: 25205458
- This research demonstrated that DNA methylation is a key mechanism underlying the silencing of NOLC1 expression in human hepatocellular carcinoma cells. Hypermethylation of the four CpG dinucleotides within the NOLC1 gene is a potential biomarker for hepatocellular carcinoma. PMID: 23970161
- A Nopp140 fragment (residues 568-596) and inositol hexakisphosphate competitively bind to the catalytic subunit of CK2 (CK2alpha). Phosphorylation at Ser574 of Nopp140 significantly enhances its interaction with CK2alpha. PMID: 24218616
- The H5N1 viral NS1 protein interacts with the host NOLC1 protein. PMID: 23188192
- This study investigated the structural disorder and local order of hNopp140. PMID: 22906532
- The regulation of NOCL1 protein expression is critically influenced by the transcription factors NF-kB and cAMP response element-binding protein. PMID: 21266110
- NOPP140 is implicated in a PKA signaling pathway that leads to the activation of agp. PMID: 12167624
- These findings indicate that Nopp140 reversibly associates with snoRNPs in a phosphorylation-dependent manner. However, this association does not affect pseudouridylation of rRNA. PMID: 12446766
- Hypoxia induces substantial dephosphorylation and nuclear accumulation of p130, leading to the formation of E2F4/p130 complexes and increased occupancy of E2F4 and p130 at the RAD51 and BRCA1 promoters. PMID: 17001309
- hNopp140 acts as a negative regulator of CK2. InsP(6) stimulates the activity of CK2 by interfering with the interaction between hNopp140 and CK2. PMID: 17038328
- An evolutionarily conserved multisubunit protein complex containing p130 and E2F4 mediates the repression of cell cycle-dependent genes during quiescence. PMID: 17531812
- hNopp140, a scaffold protein, is involved in nucleolar assembly, fusion, and maintenance. PMID: 18253863
- InsP(6) specifically binds to CK2alpha and disrupts the interaction between CK2alpha and Nopp140, with an IC(50) value of 25 microM. This disruption attenuates the Nopp140-mediated repression of CK2 activity. PMID: 18790693
- Functional deficiency in spinal muscular atrophy cells is associated with decreased localization of the snoRNP chaperone Nopp140 in Cajal bodies, which correlates with disease severity. PMID: 19129172
- Nopp140 might be a component involved in the early stages of pre-rRNA processing. PMID: 19381672
- Both NOLC1 and tumor protein 53 work synergistically to activate the MDM2 promoter in NPC cells. PMID: 19541936
Database Links
Subcellular Location
Nucleus, nucleolus. Cytoplasm.
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