NOP16 Human
Description
Molecular Characterization of NOP16 Human
NOP16 (Nucleolar Protein 16 Homolog) is a 178-amino-acid protein encoded by the NOP16 gene (Gene ID: 51491) on chromosome 5q35.2. Recombinant variants, such as His-tagged NOP16 (e.g., Cat.No. NOP16-7099H), are produced in E. coli and purified via chromatography .
The protein contains methylated lysine residues (K29, K106, K107) critical for binding epigenetic regulators like PRC2 (Polycomb Repressive Complex 2) and JMJD3 (a histone demethylase) .
Biological Functions and Mechanisms
NOP16 regulates histone H3K27 methylation by acting as a histone mimetic:
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Epigenetic Modulation:
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Cell Cycle Regulation:
Breast Cancer
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Upregulation: Driven by c-Myc transcription and estrogen signaling .
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Prognostic Marker: Overexpression correlates with poor survival (HR = 1.76, p = 0.015) .
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In Vivo Impact: NOP16 knockout in MDA-MB231 xenografts reduces tumor growth by 60% (p < 0.01) .
Hepatocellular Carcinoma (LIHC)
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Pathogenic Role:
| Cancer Type | Key Associations |
|---|---|
| Breast | c-Myc dependency; H3K27me3 dysregulation; poor survival |
| Liver (LIHC) | Keap1-Nrf2 pathway activation; T-lymphocyte infiltration imbalance |
Key Studies
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bioRxiv (2023):
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Sage Journals (2024):
Targeted Therapy Potential
Product Specs
Product Science Overview
The NOP16 gene is located on chromosome 5 in humans. It encodes a protein that is primarily localized in the nucleolus, a substructure within the nucleus responsible for ribosome production. The human recombinant NOP16 protein is typically expressed in E. coli and is a single, non-glycosylated polypeptide chain containing 201 amino acids, with a molecular weight of approximately 23.6 kDa . It is often fused to a His-tag at the N-terminus to facilitate purification.
NOP16 expression is regulated by estrogens and the MYC protein . This regulation is significant because MYC is a well-known oncogene, and its overexpression is associated with various cancers, including breast cancer. High levels of NOP16 have been correlated with poor clinical outcomes in breast cancer patients, making it a potential biomarker for prognosis .
NOP16 plays a crucial role in ribosome biogenesis, which is essential for protein synthesis and cell growth. It is involved in the maturation of the 60S ribosomal subunit, a component of the ribosome. The protein’s expression is induced by estrogens and MYC, linking it to cell proliferation and cancer progression .
The clinical significance of NOP16 lies in its potential as a biomarker for cancer prognosis. Studies have shown that high expression levels of NOP16 are associated with aggressive tumor characteristics and poor survival rates in breast cancer patients . This makes NOP16 a valuable target for cancer research and potential therapeutic interventions.
Recombinant NOP16 is produced using recombinant DNA technology, which involves inserting the NOP16 gene into a suitable expression system, such as E. coli. The recombinant protein is then purified using various techniques, including affinity chromatography, which exploits the His-tag fused to the protein . This recombinant form is used in research to study the protein’s function, structure, and role in disease.
