NPR2 Recombinant Monoclonal Antibody

The NPR2 recombinant monoclonal antibody is produced in vitro using synthetic genes. Our proprietary technology involves isolating NPR2 antibody genes from B cells of immunized rabbits, amplifying and cloning these genes into appropriate phage vectors, transfecting mammalian cell lines with these vectors, and achieving high-yield expression of functional antibody. The resulting NPR2 recombinant monoclonal antibody is purified from the cell culture supernatant via affinity chromatography. This antibody is suitable for use in ELISA, Western blotting (WB), immunofluorescence (IF), and flow cytometry (FC) applications for the detection of human NPR2 protein.

NPR2, a receptor for natriuretic peptides, plays a crucial role in regulating blood pressure, fluid balance, and electrolyte homeostasis. Its activation promotes vasodilation, natriuresis, and diuresis, contributing to cardiovascular and renal health and counteracting conditions such as hypertension and heart failure.

NPR2 is a receptor for the C-type natriuretic peptide hormone NPPC/CNP. It exhibits guanylate cyclase activity upon ligand binding and may play a significant role in regulating skeletal growth.

NPR2 Gene Function and Associated Studies:

• Atenolol treatment normalized altered expression of Npr1 and Npr2 genes. (PMID: 27283501)
• In four Indian families with acromesomelic dysplasia, Maroteaux type, four homozygous mutations were identified, including three novel mutations (a frameshift deletion, p.Cys586Ter; a nonsense mutation, p.Arg479Ter; and a missense mutation, p.Val187Asp) and one previously reported missense mutation (p.Tyr338Cys). (PMID: 27994189)
• Heterozygous mutations in the NPR2 gene are associated with short stature. (PMID: 27941173)
• Mutations in three genes (GDF5, NPR2, BMPR1B) have been implicated in different forms of acromesomelic dysplasia. (PMID: 26926249)
• IL1R2 hypomethylation and androgen receptor hypermethylation may influence disease severity in obstructive sleep apnea, while NPR2 hypomethylation and SP140 hypermethylation may serve as biomarkers for excessive daytime sleepiness. (PMID: 26888452)
• Loss-of-function mutations in the NPR2 gene are associated with acromesomelic dysplasia, Maroteaux type. (PMID: 26567084)
• Heterozygous mutations in the natriuretic peptide receptor-B (NPR2) gene are a cause of short stature. (PMID: 25703509)
• NPR2 mutations account for approximately 3% of cases of disproportionate short stature with clinical or radiographic indicators of SHOX deficiency where no SHOX defect has been identified. (PMID: 26075495)
• Three consanguineous families with acromesomelic dysplasia, Maroteaux type, showed linkage to the NPR2 gene on chromosome 9p12-21. Sequence analysis revealed two novel missense variants (p.Arg601Ser; p.Arg749Trp) and a previously reported splice site variant (c.2986+2T>G). (PMID: 25959430)
• An overgrowth syndrome is associated with a gain-of-function mutation of the NPR2 gene. (PMID: 24259409)
• Heterozygous dominant-negative NPR2 mutations were identified in 2% of Japanese patients with short stature. (PMID: 24471569)
• Kidney NPR2 protein quantity is significantly affected by genetic variation. (PMID: 23835779)
• The V883M mutation increases maximal velocity in the absence of CNP, eliminates the ATP requirement for CNP-dependent Km reduction, and disrupts normal inactivation; this establishes a molecular mechanism for how an amino acid substitution in GC-B activates the enzyme, leading to abnormally long and fragile bones. (PMID: 23827346)
• In transgenic mice, complete absence of Npr2 activity prevents the bifurcation of cranial sensory axons. (PMID: 24431432)
• Mutations in Tyr808 markedly enhanced GC-B activity. (PMID: 23586811)
• Heterozygous NPR2 mutations were identified in 6% of patients initially classified as having idiopathic short stature. Affected patients exhibit mild and variable degrees of short stature without a distinct phenotype. (PMID: 24001744)
• The extracellular domain of human GC-B folds independently of the rest of the protein. (PMID: 19108585)
• An overgrowth disorder is associated with excessive cGMP production due to a gain-of-function mutation in the NPR2 gene. (PMID: 22870295)
• Patients with BNP levels >150 pg/mL on admission have a higher probability of death in follow-up. (PMID: 22633662)
• Two novel missense mutations in the NPR2 gene were identified in six consanguineous families of Pakistani origin. The presence of the same mutation (p.T907M) and haplotype in five families suggests a founder effect. (PMID: 22691581)
• NPR2 expression in normal human fetal and adult pituitaries and adenomatous pituitary tissue suggests a role in both pituitary development and oncogenesis. (PMID: 22645228)
• GC-B activity is increased in non-myocytes from failing human ventricles, possibly due to increased fibrosis. (PMID: 22133375)
• Evidence suggests a potential causal role of the B-type natriuretic peptide system in the etiology of type 2 diabetes. (PMID: 22039354)
• Serum B-type natriuretic peptide strongly correlates with new-onset heart failure development at an optimal cut-off value of 175 pg/mL. (PMID: 20600420)
• NPR-A and NPR-B (mRNA and protein) are present in human corneal epithelial tissue. (PMID: 20664698)
• A polymorphism in natriuretic peptide receptor 2 influences susceptibility to idiopathic dilated cardiomyopathy in a Chinese cohort. (PMID: 20123316)
• VILIP-1 regulates the cell surface localization of natriuretic peptide receptor B. (PMID: 20079378)
• NPR-B is highly expressed in glomeruli and proximal tubules, while NPR-Bi (a splice variant) shows strong expression in the distal nephron. (PMID: 12709393)
• BNP is a marker for left ventricular dysfunction in diabetic patients. (PMID: 14988324)
• Mutations in the transmembrane natriuretic peptide receptor NPR-B impair skeletal growth and cause acromesomelic dysplasia, Maroteaux type. (PMID: 15146390)
• The 5' terminus of the hNPR-B gene transcript is approximately 732 base pairs upstream from the presumed translation start site. Its activity is dominated by a single cluster of Sp1-binding elements in the proximal 5' flanking sequence of the gene. (PMID: 15262909)
• Hyperosmotic and lysophosphatidic acid-dependent inhibition of NPRB is mediated by calcium-dependent phosphorylation. (PMID: 15371450)
• The role of NPR-B and its ligand C-type natriuretic peptide in cardiovascular physiology and disease. (PMID: 17429599)
• The intact kinase homology domain of NPR-B is essential for skeletal development. (PMID: 17652215)
• Defective cellular trafficking of NPR-B resulting from missense mutations is associated with acromesomelic dysplasia-type Maroteaux. (PMID: 18945719)
• BNP level on arrival in the intensive care unit may aid in the early diagnosis and optimal management of heart failure after aortic valve replacement. (PMID: 19167912)
• Subjects homozygous for the C allele at position 381 of the BNP precursor gene promoter are more prone to developing atherosclerotic lesions in renal arteries. (PMID: 19413180)
• Protein structure: ligand binding domains. (PMID: 11556325)

HGNC: 7944

OMIM: 108961

KEGG: hsa:4882

STRING: 9606.ENSP00000341083

UniGene: Hs.78518