NRBP1 Antibody
Description
Research Applications of NRBP1 Antibody
NRBP1 antibodies are widely used to investigate the protein’s role in cancer biology and signaling pathways. Key applications include:
Cancer Studies
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Triple-Negative Breast Cancer (TNBC): Detects elevated NRBP1 expression linked to poor prognosis and metastasis via Rac1/Cdc42 activation .
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Colorectal Cancer (CRC): Identifies NRBP1 as a tumor suppressor that induces caspase-dependent apoptosis .
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Bladder Cancer: Highlights NRBP1’s pro-tumorigenic role in proliferation and lymph node metastasis .
Oncogenic vs. Tumor-Suppressive Roles
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Pro-Tumorigenic: In TNBC, NRBP1 promotes metastasis via P-Rex1-mediated Rac1 activation . In bladder cancer, high NRBP1 expression correlates with advanced tumor stage .
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Tumor-Suppressive: In CRC, NRBP1 induces apoptosis through JNK/caspase-9 signaling .
Mechanistic Insights
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NRBP1 scaffolds P-Rex1 to enhance GTP-bound Rac1/Cdc42 levels, driving TNBC cell migration .
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NRBP1 knockdown in bladder cancer reduces Ki-67 (proliferation marker) and increases cleaved caspase-3 (apoptosis marker) .
Western Blotting
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Sample Preparation: Use RIPA buffer for protein extraction.
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Detection: Chemiluminescence with HRP-conjugated secondary antibodies .
Immunohistochemistry
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Staining Protocol: Heat-induced antigen retrieval, DAB chromogen, hematoxylin counterstain .
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Scoring System: Immunoreactivity score (0–12) based on staining intensity and distribution .
Limitations and Future Directions
Product Specs
Target Background
NRBP1 (Nuclear Receptor Binding Protein 1) plays a role in intracellular trafficking between the endoplasmic reticulum and Golgi apparatus. This function is mediated through interactions with Rho-type GTPases. Studies indicate that NRBP1's interaction with the dengue virus type 2 NS3 protein disrupts this trafficking, leading to alterations in intracellular membrane structure associated with viral replication.
- NRBP1 has been identified as a gout risk gene. Increased NRBP1 expression in gout patients may be regulated by methylation-dependent TFAP2A binding to a region 72 base pairs upstream of the NRBP1 transcription start site. PMID: 28932319
- Molecular modeling studies have investigated the interaction between the dengue virus (DENV1-4) NS3 protein and NRBP. Protein-protein docking studies explored this interaction. PMID: 24910013
- Homology modeling and protein-protein docking studies have examined the interaction between DENV NS3 and NRBP. These studies build on the established interaction between DENV NS3 and NRBP, which affects cellular trafficking. PMID: 24372240
- High NRBP1 expression in prostate cancer is associated with poor clinical outcomes and increased cancer cell growth. PMID: 22473923
- NRBP1 acts as a tumor suppressor crucial for intestinal cell homeostasis. Reduced NRBP1 levels are observed in various human cancers, and low levels in lung cancer correlate with poor prognosis. PMID: 22510880
- Studies of the Rac3/NRBP interaction suggest NRBP's role in subcellular trafficking, potentially targeted to specific locations through Rho family small GTPase interactions. PMID: 11956649
- NRBP1 mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. PMID: 12176995
- The dengue virus type 2 NS3 protein specifically interacts with NRBP, a host protein influencing endoplasmic reticulum-Golgi trafficking and interacting with Rac3. PMID: 15084397
