NFE2L2 Antibody
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Description
Research Applications and Validation
The NFE2L2 antibody has been extensively validated across diverse experimental contexts:
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Disease Studies: Used to investigate NRF2's role in oxidative stress-related pathologies, including diabetic retinopathy, Alzheimer’s disease, and non-small cell lung cancer, where NRF2 dysregulation promotes therapeutic resistance .
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Mechanistic Insights: Facilitates exploration of NRF2-KEAP1 interactions, which regulate cellular antioxidant responses. Under oxidative stress, NRF2 escapes KEAP1-mediated degradation, translocates to the nucleus, and activates cytoprotective genes .
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Single-Cell Analysis: Employed in ankylosing spondylitis (AS) research to identify elevated NRF2 expression in neutrophils, suggesting its role in ferroptosis and inflammation .
Key Publications (as of March 2025):
| Application | Number of Citations |
|---|---|
| Western Blot | 1,727 |
| Immunohistochemistry | 244 |
| Immunofluorescence | 450 |
| ChIP/CoIP | 20–33 |
Functional Relevance in Disease Contexts
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ARDS and Sepsis: Genetic variants of NFE2L2 (e.g., rs672961) are linked to acute respiratory distress syndrome (ARDS) susceptibility in sepsis patients. The antibody aids in studying NRF2’s regulatory role in oxidative stress responses during critical illness .
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Cancer Therapeutics: In non-small cell lung cancer, NRF2 overexpression correlates with poor prognosis. The antibody helps assess NRF2 as a biomarker for drug resistance and metastasis .
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Immunopathology: Used to analyze NRF2’s interaction with immune cells, such as neutrophils in AS, where its upregulation exacerbates inflammatory pathways .
Technical Considerations
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Specificity: The antibody recognizes both native and denatured forms of NRF2, confirmed by knockdown/knockout validation in over 150 studies .
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Cross-Reactivity: Detects NRF2 across multiple species, including primates and avian models, broadening its utility in comparative studies .
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Artifact Mitigation: Researchers must account for post-translational modifications (e.g., ubiquitination, phosphorylation) that alter NRF2’s molecular weight and subcellular localization .
Product Specs
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Description
The CSB-PA003481 NFE2L2 polyclonal antibody was produced in rabbits immunized with a synthesized peptide derived from the C-terminal region of Human Nrf2. NFE2L2, also known as Nuclear factor (erythroid-derived 2)-like 2, is a transcription factor that plays a critical role in the cellular response to oxidative stress. It binds to antioxidant response (ARE) elements located within the promoter regions of numerous cytoprotective genes, including phase 2 detoxifying enzymes. This binding event promotes the expression of these genes, ultimately contributing to the neutralization of reactive electrophiles. Diseases associated with NFE2L2 include Immunodeficiency, Developmental Delay, and Hypohomocysteinemia, as well as Lung Squamous Cell Carcinoma.
This NFE2L2 Antibody has been rigorously tested in ELISA, WB, IF, and IHC assays. The non-conjugated IgG underwent purification using protein G, achieving a purity level of 95%. It exhibits reactivity with NFE2L2 proteins of human, mouse, or rat origin. This antibody can be effectively utilized to detect endogenous levels of NFE2L2 protein.
This NFE2L2 Antibody has been rigorously tested in ELISA, WB, IF, and IHC assays. The non-conjugated IgG underwent purification using protein G, achieving a purity level of 95%. It exhibits reactivity with NFE2L2 proteins of human, mouse, or rat origin. This antibody can be effectively utilized to detect endogenous levels of NFE2L2 protein.
Form
Liquid
Lead Time
Typically, we can dispatch products within 1-3 business days following receipt of your orders. Delivery timelines may vary depending on the purchasing method or location. For specific delivery information, kindly consult your local distributors.
Synonyms
erythroid derived 2 antibody; HEBP1 antibody; like 2 antibody; NF E2 related factor 2 antibody; NF-E2-related factor 2 antibody; NF2L2_HUMAN antibody; NFE2 related factor 2 antibody; NFE2-related factor 2 antibody; Nfe2l2 antibody; Nrf 2 antibody; NRF2 antibody; Nuclear factor (erythroid derived 2) like 2 antibody; Nuclear factor antibody; nuclear factor erythroid 2 like 2 antibody; Nuclear factor erythroid 2 related factor 2 antibody; Nuclear factor erythroid 2-related factor 2 antibody; Nuclear factor erythroid derived 2 like 2 antibody
Target Names
Uniprot No.
Target Background
Function
NFE2L2, a critical transcription factor in the cellular response to oxidative stress, binds to antioxidant response (ARE) elements within promoter regions of various cytoprotective genes, such as phase 2 detoxifying enzymes. This binding event promotes the expression of these genes, effectively neutralizing reactive electrophiles. Under normal physiological conditions, NFE2L2 is ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex. However, upon exposure to oxidative stress, electrophile metabolites inhibit the activity of the BCR(KEAP1) complex, facilitating the nuclear accumulation of NFE2L2. This nuclear translocation leads to heterodimerization with one of the small Maf proteins, subsequently binding to ARE elements of cytoprotective target genes. The NFE2L2 pathway is also activated in response to selective autophagy. Autophagy promotes interaction between KEAP1 and SQSTM1/p62, resulting in inactivation of the BCR(KEAP1) complex. This inactivation triggers NFE2L2 nuclear accumulation and the expression of cytoprotective genes.
NFE2L2 may also play a role in the transcriptional activation of genes within the beta-globin cluster by mediating enhancer activity at the hypersensitive site 2 of the beta-globin locus control region. Additionally, NFE2L2 plays a crucial role in regulating the innate immune response and antiviral cytosolic DNA sensing. It serves as a critical regulator of the innate immune response and promotes survival during sepsis by maintaining redox homeostasis and restraining the dysregulation of proinflammatory signaling pathways, such as MyD88-dependent and -independent and TNF-alpha signaling.
NFE2L2 effectively suppresses macrophage inflammatory response by inhibiting the transcription of proinflammatory cytokines and the induction of IL6. It binds to the proximity of proinflammatory genes in macrophages and inhibits RNA Pol II recruitment, a process independent of the NRF2-binding motif and reactive oxygen species level. NFE2L2 represses antiviral cytosolic DNA sensing by suppressing the expression of the adapter protein STING1, leading to decreased responsiveness to STING1 agonists and increased susceptibility to infection with DNA viruses.
Upon activation, NFE2L2 effectively limits the release of pro-inflammatory cytokines in response to human coronavirus SARS-CoV-2 infection and to virus-derived ligands. This regulation is mediated by a mechanism involving the inhibition of IRF3 dimerization. Additionally, NFE2L2 inhibits both SARS-CoV-2 replication and the replication of several other pathogenic viruses, including Herpes Simplex Virus-1 and -2, Vaccinia virus, and Zika virus through a type I interferon (IFN)-independent mechanism.
NFE2L2 may also play a role in the transcriptional activation of genes within the beta-globin cluster by mediating enhancer activity at the hypersensitive site 2 of the beta-globin locus control region. Additionally, NFE2L2 plays a crucial role in regulating the innate immune response and antiviral cytosolic DNA sensing. It serves as a critical regulator of the innate immune response and promotes survival during sepsis by maintaining redox homeostasis and restraining the dysregulation of proinflammatory signaling pathways, such as MyD88-dependent and -independent and TNF-alpha signaling.
NFE2L2 effectively suppresses macrophage inflammatory response by inhibiting the transcription of proinflammatory cytokines and the induction of IL6. It binds to the proximity of proinflammatory genes in macrophages and inhibits RNA Pol II recruitment, a process independent of the NRF2-binding motif and reactive oxygen species level. NFE2L2 represses antiviral cytosolic DNA sensing by suppressing the expression of the adapter protein STING1, leading to decreased responsiveness to STING1 agonists and increased susceptibility to infection with DNA viruses.
Upon activation, NFE2L2 effectively limits the release of pro-inflammatory cytokines in response to human coronavirus SARS-CoV-2 infection and to virus-derived ligands. This regulation is mediated by a mechanism involving the inhibition of IRF3 dimerization. Additionally, NFE2L2 inhibits both SARS-CoV-2 replication and the replication of several other pathogenic viruses, including Herpes Simplex Virus-1 and -2, Vaccinia virus, and Zika virus through a type I interferon (IFN)-independent mechanism.
Gene References Into Functions
- The key antioxidant enzymes and transcription factor Nrf2 are up-regulated, and the NOX5 expression is reduced during the development of drug resistance of tumor cells to cisplatin. PMID: 30225719
- HIF1A is upregulated in breast and bladder tumors with high NRF2 activity. NRF2 targets a functional antioxidant response element at the HIF1A locus, revealing a direct regulatory connection between these two significant oxygen-responsive transcription factors. PMID: 30241031
- A core set of 32 direct NRF2 targets are consistently upregulated in NRF2 hyperactivated tumors. PMID: 30195190
- The expression of Nrf2-ARE molecules and related antioxidases is significantly decreased in patients with OSAHS and is correlated with neurocognitive dysfunction. PMID: 30159112
- These findings suggest that constitutive activation of Nrf2 might be linked to tumor aerobic glycolysis and progression of UTUC, indicating that Nrf2 signaling in the tumor microenvironment promotes progression of UTUC. PMID: 29716554
- NRF2 is a transcription factor that robustly transduces chemical signals to regulate a battery of cytoprotective genes. KEAP1 represses NRF2 activity under quiescent conditions, whereas NRF2 is liberated from KEAP1-mediated repression upon exposure to stresses. [review] PMID: 29717933
- The ability of porphyra-334 and shinorine to dissociate Nrf2 from Keap1 was confirmed also by measurement of increased mRNA expression of Nrf2 targeted genes encoding oxidative stress defense proteins in primary skin fibroblasts prior and post UVR exposure. PMID: 30071261
- NRF2, DJ1 and SRNX1 are commonly expressed in diffusely infiltrating astrocytomas and they can be used in predicting patient prognosis. PMID: 29441509
- The present findings were the first to show that pterostilbene attenuated high glucose-induced central nervous system injury in vitro through the activation of Nrf2 signaling, displaying protective effects against mitochondrial dysfunction-derived oxidative stress. PMID: 28089584
- In some breast cancer cells, xCT antiporter expression is upregulated through the antioxidant transcription factor Nrf2 and contributes to their requirement for glucose as a carbon source. PMID: 28429737
- This study provides support for the involvement of both NFE2L2 and PPARGC1alpha in Parkinson's disease susceptibility and progression, marginally and through pathways involving maneb and paraquat exposure. PMID: 29630901
- Our data suggests a novel interaction between Nrf2 and ATF4 under oxidative and endoplasmic reticulum stress, thus driving specific enzymatic and non-enzymatic reactions of antioxidant mechanisms maintaining redox homeostasis. PMID: 29421327
- The senescence-associated downregulation of NRF2 decreases endothelial glycolytic activity and stress tolerance, both of which are restored after reinstating NRF2. PMID: 29986211
- Aberrant Nrf2/Keap1 system integrity may affect self-defense mechanisms against oxidative stress in primary biliary cholangitis. PMID: 28333129
- Inflammation, oxidative stress, and higher expression levels of Nrf2 and NQO1 proteins in the airways of women chronically exposed to biomass fuel smoke. PMID: 29363060
- Data suggest that NRF2/NFE2L2 promotes breast cancer progression by enhancing glycolysis through co-activation of HIF1A; NRF2 and HIF1A mRNA and protein levels are significantly up-regulated in breast cancer cells as compared to benign breast epithelial cells. (NRF2/NFE2L2 = nuclear factor erythroid 2-related factor 2; HIF1A = hypoxia inducible factor 1 subunit alpha) PMID: 29275212
- Results show that NRF2 expression is regulated by NRG1 in papillary thyroid cancer (PTC). PMID: 29901070
- 27-OH induced autophagy is dependent on the relationship between nuclear factor erythroid 2 p45-related factor 2 (Nrf2)-dependent antioxidant response and p62. PMID: 29879549
- Evidence for a direct role of NRF2 in globin gene regulation PMID: 28473619
- The CD44-NRF2 axis might be a promising therapeutic target for the control of stress resistance and survival of CD44(high) CSC population within breast tumors. PMID: 29729523
- This study shows changes of NRF2 expression levels induced by cell-free DNA in different cell types PMID: 29743966
- Metabolism-dependent clonal growth of HCT15 colorectal cancer cells was induced by Nrf2-dependent activation of MCT1-driven lactate exchange. PMID: 28846107
- Here we present a proof-of-concept application for the rational design of an epitope-specific antibody binding with the target protein Keap1, by grafting pre-defined structural interaction patterns from the native binding partner protein, Nrf2, onto geometrically matched positions of a set of antibody scaffolds. The designed antibodies bind to Keap1 and block the Keap1-Nrf2 interaction in an epitope-specific way PMID: 28128368
- This review focuses on some fundamental aspects of Nrf2 effects on redox systems, mitochondrial function, and proteostasis. [Review Article] PMID: 28424271
- Therapeutic inhibition of Nrf2/ABCB1 signaling can be considered as a novel strategy. PMID: 29793178
- The cross-talk between HIF1A, NRF2 and NF-kappaB is required to adapt to changes in oxygen availability. (Review) PMID: 29485192
- The effect of lutein antiproliferation was mediated by activation of the NrF2/ARE pathway, and blocking of the NF-kappaB signaling pathway..lutein treatment decreased NF-kappaB signaling pathway related NF-kappaB p65 protein expression PMID: 29336610
- Nuclear factor erythroid-2-related factor regulates LRWD1 expression and cellular adaptation to oxidative stress in human embryonal carcinoma cells PMID: 29544732
- In human keratinocytes, authors found that perillaldehyde (1) inhibited BaP-induced AHR activation and ROS production, (2) inhibited BaP/AHR-mediated release of the CCL2 chemokine, and (3) activated the NRF2/HO1 antioxidant pathway. PMID: 29643980
- Authors show that NRF2 regulates both basal and inducible expression of HER1, as treatment of ovarian cancer cells (PEO1, OVCAR3, and SKOV3) with NRF2 activator tBHQ inducing HER1, while inhibition of NRF2 by siRNA knockdown or with retinoid represses HER1. PMID: 29410730
- Nrf2 deregulation has been linked to both aging and pathogenesis of many human chronic diseases, including neurodegenerative ones such as Parkinson's disease, Alzheimer's disease and amyotrophic lateral sclerosis. Nrf2 activity is tightly regulated by a fine balance between positive and negative modulators. PMID: 29969760
- our results demonstrate that Pc-induced expression of HO-1 is mediated by the PKCA-Nrf-2/HO-1 pathway, and inhibits UVB-induced apoptotic cell death in primary skin cells. PMID: 29470442
- our study emphasizes the discovery of a gene signature regulated by the KEAP1-NRF2-CUL3 axis which is strongly associated with tumorigenesis and drug resistance in head and neck squamous cell cancer . PMID: 29306329
- Nrf2 overexpression was associated with tumor size, histological grade, and metastasis in gastric cancer patients. PMID: 29091877
- Data (including data from studies using tissues/cells from transgenic mice) suggest that ChREBPalpha up-regulates expression and activity of NRF2, initiating mitochondrial biogenesis in beta-cells; induction of NRF2 is required for ChREBPalpha-mediated effects and for glucose-stimulated beta-cell proliferation. [ChREBP = carbohydrate-responsive element-binding protein] PMID: 29764859
- The NFE2L2 promotor variant rs6721961 may be protective against hearing loss in cisplatin-receiving cancer patients by affecting NFE2L2 gene expression. PMID: 27457817
- Following arachidin-1 pretreatment, the H2O2-induced generation of reactive oxygen species was reduced. Therefore, the present results indicate that arachidin-1 suppresses TNF-alpha-induced inflammation in ECs through the upregulation of Nrf-2-related phase II enzyme expression. PMID: 29115410
- In the present review, we briefly introduce the Nrf2-Keap1 system and describe Nrf2 functions, illustrate the Nrf2-NF-kappaB cross-talk, and highlight the effects of the Nrf2-Keap1 system in the physiology and pathophysiology of striated muscle tissue taking into account its role(s) in oxidative stress and reductive stress PMID: 29499228
- In light of a pivotal role of NRF2 and BACH1 in response to oxidative stress and regulation of HO-1, we examined if smoke-induced HO-1 expression is modulated through the NRF2/BACH1 axis. We demonstrated that smoke causes significant nuclear translocation of NRF2, but only a slight decrease in nuclear BACH1. PMID: 29125538
- Study found that NRF2 expression level was significantly down-regulated in the hepatic ischemia-reperfusion (HIR) model. PMID: 28708282
- Enhanced BRAF-mediated NRF2 gene transcription and Histone Acetyltransferases-mediated NRF2 protein acetylation contributes to ABCC1-mediated chemoresistance and glutathione-mediated survival in acquired topoisomerase II poison-resistant cancer cells. PMID: 29080842
- Respiratory syncytial virus induces NRF2 degradation through a PML-RNF4 pathway. PMID: 29107745
- Results show that NRF2 activity is positively regulated by miR-432-3p through downregulation of KEAP1 inducing its stability in squamous cell carcinoma. PMID: 28760781
- An intact complex of PGAM5-KEAP1-Nrf2 preserves mitochondrial motility by suppressing dominant-negative KEAP1 activity. PMID: 28839075
- Hydrogen sulfide attenuates vascular smooth muscle cell calcification in vitro via the KEAP1-NRF2 redox sensing/stress response system by enhancing NQO1 expression. PMID: 28865326
- 5-hydroxyeicosatetraenoic acid and 5-hydroxyeicosapentaenoic acid were converted to 5-oxo-eicosatetraenoic acid (5-oxo-ETE) in HUVECs, and 5-oxo-ETE increased Nrf2 activation. These results suggest that 5-hydroxyeicosatetraenoic acid and 5-hydroxyeicosapentaenoic acid works as an Nrf2 activator through the metabolite 5-oxo-ETE in HUVECs. PMID: 28892009
- The interplay between Nrf2 and VEGF can contribute to venous hypertension-induced angiogenesis in brain arteriovenous malformations pathogenesis. PMID: 27869147
- High Nrf2 expression in alveolar type I pneumocytes is associated with low recurrences in primary spontaneous pneumothorax. PMID: 28962820
- Here, the authors show that activation of NRF2, in either mouse or human cancer cells, leads to increased dependency on exogenous glutamine through increased consumption of glutamate for glutathione synthesis and glutamate secretion by xc(-) antiporter system. PMID: 28967864
- PCR and western blot results showed that inhibiting the secretion of Wnt3A blocked the Wnt signaling pathway and prevented Nrf2 signaling. Notably, the Wnt inhibitor may serve as a radiosensitizing drug PMID: 28627706
Database Links
Protein Families
BZIP family, CNC subfamily
Subcellular Location
Cytoplasm, cytosol. Nucleus.
Tissue Specificity
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Customer Reviews
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B.A
By Anonymous
★★★★★
Applications : WB
Sample type: Human UCB-MSCs
Review: Cells were transfected with ERα siRNA or NT siRNA for 24 h prior to high glucose treatment for 72 h where nuclear fraction was carried out. Nuclear Nrf2 was detected by western blot. Lamin A/C was used as the nuclear marker.
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