NSF Antibody

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Cat. No.
BT1248878
Synonyms
NSF antibody; At4g04910 antibody; T1J1.4 antibody; Vesicle-fusing ATPase antibody; EC 3.6.4.6 antibody; N-ethylmaleimide-sensitive fusion protein antibody; Vesicular-fusion protein NSF antibody
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Description

Definition and Target

NSF antibodies are polyclonal or monoclonal antibodies that bind specifically to the NSF protein. NSF is a hexameric ATPase essential for disassembling SNARE (soluble NSF-attachment protein receptor) complexes, enabling vesicle fusion and neurotransmitter release .

Research Applications

NSF antibodies are widely used in:

ApplicationDetails
Western BlottingDetects NSF at ~83 kDa in brain tissue (mouse, rat, human) .
ImmunoprecipitationIsolates NSF-interacting proteins (e.g., SNAREs, viral proteins) .
ImmunofluorescenceLocalizes NSF to the Golgi apparatus, cytosol, and plasma membrane .
Disease StudiesInvestigates NSF’s role in Parkinson’s disease, Alzheimer’s, and epilepsy .

Synaptic Function

  • NSF antibodies revealed that NSF depletion increases docked synaptic vesicles, impairing neurotransmitter release .

  • NSF interacts with AMPA receptors (e.g., GRIA2) to regulate their membrane recycling .

Neurological Disorders

  • Parkinson’s Disease: NSF aggregation due to LRRK2 phosphorylation correlates with dopaminergic neuron loss .

  • Alzheimer’s Disease: Tau protein binding disrupts NSF localization, contributing to memory deficits .

Viral Replication

  • NSF antibodies identified NSF’s role in baculovirus nuclear egress, where dominant-negative NSF mutants inhibit viral replication .

Future Directions

  • Structural Studies: High-resolution imaging of NSF domains to elucidate SNARE dissociation mechanisms .

  • Therapeutic Targets: Screening small-molecule NSF modulators to mitigate neurodegenerative pathologies .

  • Viral Inhibition: Targeting NSF-viral protein interactions to block viral assembly .

Product Specs

Buffer
**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
NSF antibody; At4g04910 antibody; T1J1.4 antibody; Vesicle-fusing ATPase antibody; EC 3.6.4.6 antibody; N-ethylmaleimide-sensitive fusion protein antibody; Vesicular-fusion protein NSF antibody
Target Names
NSF
Uniprot No.
Q9M0Y8

Target Background

Function
NSF Antibody is a critical component of vesicle-mediated transport. It catalyzes the fusion of transport vesicles within the Golgi cisternae, and is also essential for transport from the endoplasmic reticulum to the Golgi stack. NSF functions as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack, irrespective of vesicle origin. It plays a vital role in maintaining the normal morphology of the Golgi apparatus.
Gene References Into Functions
  1. The missense mutation D374N in the NSF gene, encoding the N-ethylmaleimide-sensitive factor, has been linked to a key component of membrane fusion. PMID: 29398689
Database Links

KEGG: ath:AT4G04910

STRING: 3702.AT4G04910.1

UniGene: At.33942

Protein Families
AAA ATPase family
Subcellular Location
Cytoplasm.

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