NSUN2 Antibody, HRP conjugated
Description
Definition and Mechanism
The NSUN2 Antibody, HRP conjugated is a recombinant or polyclonal antibody directed against the NSUN2 protein, covalently linked to horseradish peroxidase (HRP). This conjugation enables enzymatic detection in immunoassays, such as ELISA, Western blot, and immunohistochemistry (IHC), by converting chromogenic substrates (e.g., DAB) into visible signals. NSUN2 is an RNA methyltransferase responsible for 5-methylcytosine (m5C) modifications, critical for RNA stability and translation. Its overexpression is implicated in cancers like nasopharyngeal carcinoma (NPC), gastric cancer (GC), and retinoblastoma (RB) .
Key Techniques
| Application | Workflow | HRP Role |
|---|---|---|
| ELISA | Detects NSUN2 levels in lysates or serum. | HRP catalyzes colorimetric reactions (e.g., TMB oxidation). |
| Western Blot (WB) | Identifies NSUN2 protein bands via electrophoresis and membrane transfer. | HRP-conjugated secondary antibodies amplify primary antibody signals. |
| IHC | Visualizes NSUN2 localization in tissue sections. | HRP-DAB reaction produces brown precipitates for microscopic analysis. |
Example: In NPC studies, HRP-conjugated antibodies (e.g., secondary antibodies) are used to detect primary anti-NSUN2 signals, revealing its overexpression in tumor tissues and correlation with poor prognosis .
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Cusabio’s CSB-PA626626LB01HU is a polyclonal HRP-conjugated antibody optimized for ELISA, with a recommended dilution of 1:500–1:1000 .
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Proteintech’s 82894-9-PBS and 82894-1-PBS are conjugation-ready antibodies; HRP conjugation must be performed by the user for applications like ELISA or mass cytometry .
Oncological Insights
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Nasopharyngeal Carcinoma (NPC):
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Gastric Cancer (GC):
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Retinoblastoma (RB):
Diagnostic and Therapeutic Implications
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Biomarker Utility: NSUN2 levels detected via HRP-conjugated antibodies may predict immunotherapy responsiveness (e.g., PD-1 inhibitors) and chemotherapy sensitivity .
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Drug Development: NSUN2 inhibitors (e.g., MY-1B) show synergistic effects with PD-1 blockade, as validated using HRP-based viability assays .
Optimization and Limitations
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Antigen Retrieval: For IHC, protocols often require TE buffer (pH 9.0) or citrate buffer (pH 6.0) to unmask NSUN2 epitopes .
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Cross-Reactivity: Rabbit-derived antibodies (e.g., Proteintech’s 20854-1-AP) may require blocking agents to minimize non-specific binding .
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Storage Stability: HRP-conjugated antibodies are sensitive to freeze-thaw cycles; -20°C/-80°C storage is critical .
Product Specs
Target Background
- Elevated NSUN2 expression correlates with ovarian cancer. PMID: 28829218
- High NSUN2 levels are associated with approximately 22 months shorter overall survival and increased mortality risk (p-trend = 0.020). PMID: 29775108
- Restoration of wild-type, but not methyltransferase-deficient, NSUN2 rescues ALYREF-mediated mRNA export defects following NSUN2 depletion. PMID: 28418038
- m6A methylation by METTL3/METTL14 and m5C methylation by NSUN2 are synergistic, enhancing p21 expression at the translational level. PMID: 28247949
- NSUN2-mediated mRNA methylation plays a critical role in promoting premature senescence. PMID: 26992231
- NSUN2 overexpression exhibits significant effects in breast cancer cells. PMID: 27447970
- YB-1 and NSUN2 may mediate the sorting of specific mRNAs into exosomes, potentially through recognition of specific motifs. PMID: 28341602
- NSun2-mediated mRNA methylation regulates p27 and CDK1 levels during replicative senescence. PMID: 26687548
- A homozygous variant (c.1020delA) in NSUN2 causes a frameshift and premature stop codon, reducing mRNA levels and leading to intellectual disability. PMID: 26055038
- NSun2 methylates CDK1 mRNA at the 3'UTR, enhancing CDK1 translation and influencing cell cycle progression. PMID: 26391950
- tRNA modifying enzymes NSUN2 and METTL1 influence 5-fluorouracil sensitivity in HeLa cells. PMID: 25233213
- Short tandem repeat markers linked to TUSC3 (MRT7) or NSUN2 (MRT5) genes are used for homozygosity mapping of recessive intellectual disability. PMID: 26427135
- NSun2 methylates pri-miR-125b, pre-miR-125b, and mature miR-125b in vitro and in vivo. PMID: 25047833
- Deficiencies in NSun2-mediated tRNA methylation contribute to human diseases through stress-induced RNA cleavage. PMID: 25063673
- Impaired processing of vault ncRNA may contribute to NSun2-deficiency disorders. PMID: 23871666
- A homozygous splice mutation in NSUN2 was identified in a family with Dubowitz syndrome features. PMID: 22577224
- NSun2, a tRNA methyltransferase, inhibits p16(INK4) mRNA turnover by methylating its 3'UTR. PMID: 22395603
- Increased NSUN2 gene copy number and protein expression are associated with cancers. PMID: 22136356
- NSUN2 deficiency causes intellectual disability. PMID: 22541559
- A G679R substitution in NSUN2 impairs its nucleolar localization, causing autosomal-recessive intellectual disability. PMID: 22541562
- Extensive copy number gain and increased mRNA/protein levels of NSUN2 were observed in breast cancer cell lines and tumors. PMID: 19740597
- Intron-dependent methylation of human pre-tRNA Leu(CAA) and identification of the human Trm4 gene (encoding the responsible tRNA methylase) are reported. PMID: 17071714
- Aurora-B regulates the assembly of nucleolar RNA-processing machinery and NSUN2's methyltransferase activity through Ser139 phosphorylation during mitosis. PMID: 17215513
- c-Myc promotes proliferation by stabilizing the mitotic spindle through NSUN2 and NuSAP. PMID: 19596847
