NUP133 Antibody

Characteristics of NUP133 Antibody

NUP133 Antibody is available in multiple formats, including monoclonal and polyclonal variants, with diverse host species and specificities. Table 1 summarizes key characteristics of commercially available antibodies:

Key Notes:

• Reactivity: Most antibodies target human, mouse, and rat Nup133, with some cross-reactivity in Xenopus and yeast models .

• Purification: Antibodies range from unpurified supernatant (e.g., OAPC00044) to affinity-purified forms (e.g., 12405-1-AP) .

• Concentration: Typically 0.25–1 mg/mL, optimized for dilution in specific assays (e.g., IF: 1:50–1:100; WB: 1:1000–1:2000) .

Applications in Research

NUP133 Antibody is employed in diverse experimental workflows:

Western Blotting (WB)

• Detection: Identifies Nup133 in whole-cell lysates or immunoprecipitated complexes.

• Key Findings:

  • Observed molecular weight: ~129–133 kDa .

  • Used to study Nup133 interactions with other nucleoporins (e.g., Nup98, Nup153) .

Immunofluorescence (IF)

• Localization: Visualizes Nup133 at nuclear pore complexes and kinetochores during mitosis .

• Protocol:

  • Fixation: Paraformaldehyde or Triton X-100 permeabilization .

  • Staining: Punctate nuclear rim fluorescence in digitonin-treated cells .

Immunoprecipitation (IP)

• Complex Analysis: Isolates Nup133-bound proteins, including Sec13 and Nup160 subunits .

Functional Studies

• RNA Export: Antibodies blocking Nup133 disrupt poly(A)+ RNA export without affecting protein transport .

• Nuclear Pore Assembly: CRISPR-edited podocytes lacking Nup133 show defective pore assembly and cytoskeleton regulation .

Research Findings and Biological Roles

Nup133 is integral to nuclear pore function and cellular processes. Key studies highlight its roles:

Nuclear Pore Complex (NPC) Assembly

• Structural Role: Nup133 anchors the Nup160 complex (Nup160, Nup96, Nup107) to the nuclear envelope, ensuring proper NPC formation .

• Defective Assembly: Nup133 knockout in murine embryonic stem cells reduces Tpr-containing pores and disrupts nuclear basket integrity .

mRNA Export Regulation

• Functional Impact: Nup133 and Nup160 subunits are required for poly(A)+ RNA export. Dominant-negative fragments block mRNA export but spare protein import .

Podocyte Function and Disease

• NUP133-Linked Nephropathy: Mutations in NUP133 cause steroid-resistant nephrotic syndrome (SRNS) by impairing podocyte transcriptome regulation and actin dynamics .

• Pathogenic Mutations: Disease-associated variants reduce Nup133 protein levels or disrupt interactions with the Y-complex, leading to partial loss-of-function .

T-Cell Signaling

• Isoform-Specific Roles: Nup133 isoform 2 is implicated in Th2 signaling, linking T-cell receptor activation to c-Maf transcription factor activity .

Comparative Analysis of NUP133 Antibody Performance

Table 2 contrasts antibody efficacy in key experimental systems:

Key Trends: