NUP155 Antibody

Introduction to NUP155 Antibody

NUP155 (Nucleoporin 155) is a critical component of the nuclear pore complex (NPC), essential for nuclear envelope assembly and nucleocytoplasmic transport. The NUP155 antibody is a research tool used to detect and study this protein in diverse biological contexts, including cancer, cardiovascular diseases, and nuclear biology. It is employed in techniques like Western blotting (WB), immunohistochemistry (IHC), and immunoprecipitation (IP) to investigate NUP155’s role in cellular processes and disease mechanisms.

Validation and Performance of NUP155 Antibody

NUP155 antibodies have been rigorously validated in diverse experimental systems:

Western Blotting (WB)

• Proteintech 66359-1-Ig/1-PBS: Detects NUP155 in HEK293, HeLa, and ROS1728 cells .

• Abcam ab157104: Identifies NUP155 in 293T, HeLa, Jurkat, and NIH 3T3 lysates .

• Abcam ab233079: Confirms expression in HEK-293T and HeLa cells .

Immunohistochemistry (IHC)

• Proteintech 66359-1-Ig: Validates NUP155 expression in human breast cancer tissue, requiring antigen retrieval with TE buffer (pH 9.0) or citrate buffer (pH 6.0) .

Immunoprecipitation (IP)

• Abcam ab157104: Successfully immunoprecipitates NUP155 from 293T lysates, confirming target binding .

Research Applications of NUP155 Antibody

NUP155 antibodies have elucidated the protein’s role in disease and cellular biology:

Cancer Research

Cardiovascular Disease

• NUP155 mutations (e.g., R391H) are linked to atrial fibrillation, though antibody studies in this context remain limited .

Nuclear Biology

• Cryo-EM studies using polyclonal antibodies targeting the C-terminus have resolved NUP155’s structural role in NPC assembly .

Table 1: Antibody Validation in WB

Table 2: NUP155 Antibody in Cancer Research