NUP98B Antibody

NUP98 and Its Biological Role

NUP98 is a GLFG-domain nucleoporin essential for RNA export, transcriptional regulation, and nucleocytoplasmic transport . It localizes to both the NPC and nuclear interior, dynamically interacting with RNA and transport factors .

3.1. RNA Export and Transcriptional Regulation

• NUP98 inhibition via nuclear-injected antibodies blocks export of mRNA, snRNA, and rRNA but not tRNA .

• p21 mRNA interaction: NUP98 binds the 3′-UTR of p21, enhancing its expression. Overexpression of NUP98 increases p21 levels 10-fold compared to constructs lacking the 3′-UTR .

• GLFG-body dynamics: NUP98 associates with nuclear structures (GLFG bodies) and requires transcription for mobility .

3.2. Cancer and Apoptosis

• NUP98-96 knockdown in Drosophila induces apoptosis and JNK signaling activation, mimicking cancer-related pathways .

• NUP98 fusions (e.g., NUP98-HOXA9) are linked to leukemia, with dysregulated apoptosis and JAK/STAT signaling .

3.3. Antibody Validation and Cross-Reactivity

• 13C2 detects NUP98 in S. pombe and S. cerevisiae, recognizing FGxxN motifs conserved across species .

• 2H10 shows punctate nuclear rim staining and reacts with human, mouse, and rat NUP98 .

• EPR6678 is human-specific, used in IHC and IF for NUP98 localization .

Diagnostic and Therapeutic Potential

• Leukemia biomarker: NUP98 antibodies may aid in detecting NUP98-fusion proteins in hematopoietic malignancies .

• NPC assembly studies: Antibodies like 2H10 and 13C2 are critical for analyzing NPC dynamics and NUP98 interactions .