NUDT20 Antibody

Introduction to NUDT20 Protein

NUDT20, also known as DCP2 (mRNA-decapping enzyme 2), is a critical enzyme involved in mRNA degradation pathways. It belongs to the Nudix hydrolase family and functions as a decapping metalloenzyme that catalyzes the removal of the 7-methyl guanine cap structure from mRNA molecules . This process yields a 5'-phosphorylated mRNA fragment and 7m-GDP, which represents a crucial step in mRNA degradation pathways .

NUDT20/DCP2 is also known by several alternative names in scientific literature, including:

• m7GpppN-mRNA hydrolase

• Nucleoside diphosphate-linked moiety X motif 20

• Nudix motif 20

• hDpc

Enzymatic Activity

NUDT20/DCP2 demonstrates specific substrate preferences and activity patterns. The enzyme exhibits higher activity toward mRNAs lacking a poly(A) tail but shows no activity against cap structures without an RNA moiety . This selectivity suggests a specialized role in targeted mRNA decay rather than indiscriminate degradation of capped RNA species.

Interestingly, the presence of N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping . This finding highlights the complex regulatory mechanisms governing mRNA stability and decay.

Biological Pathways

NUDT20/DCP2 participates in several critical cellular processes:

1. It is essential for mRNA degradation in both normal mRNA turnover and nonsense-mediated mRNA decay .

2. It plays a significant role in replication-dependent histone mRNA degradation .

3. It functions as a regulatory element in autophagy by repressing the expression of ATG-related genes through degradation of their transcripts under nutrient-rich conditions .

These diverse functions position NUDT20/DCP2 as a multifunctional enzyme with important roles beyond simple mRNA decapping, suggesting its potential involvement in various cellular regulatory networks.

Antibody Conjugates and Modifications

CUSABIO offers the DCP2 antibody in various conjugated forms to accommodate different experimental applications:

Table 2: NUDT20/DCP2 Antibody Conjugates from CUSABIO

This variety of conjugated forms provides researchers with flexible options to select the most appropriate antibody format for their specific experimental requirements.

Experimental Applications

The available NUDT20/DCP2 antibodies have been validated for several laboratory techniques:

1. Immunoprecipitation (IP): The Abcam antibody (ab245661) is reported to be suitable for immunoprecipitation studies, allowing researchers to isolate and analyze NUDT20/DCP2 protein complexes .

2. Western Blotting (WB): The Abcam antibody (ab245661) has been validated for Western blotting applications, enabling the detection and quantification of NUDT20/DCP2 in protein samples .

3. Immunohistochemistry (IHC): The CUSABIO antibody (CSB-PA810265LA01HU) is recommended for IHC applications at dilutions of 1:20-1:200, facilitating the localization and visualization of NUDT20/DCP2 in tissue sections .

4. ELISA: The HRP-conjugated and Biotin-conjugated variants from CUSABIO are specifically designed for enzyme-linked immunosorbent assay applications .

Recommended Protocols

For optimal results with NUDT20/DCP2 antibodies:

• For IHC applications: The recommended dilution range for the CUSABIO antibody is 1:20-1:200 .

• For Western blotting: The Abcam antibody has been validated for detecting human samples .

Molecular Identification and Database Resources

NUDT20/DCP2 is cataloged in several biological databases with the following identifiers:

Table 3: Database Identifiers for NUDT20/DCP2

These database entries provide valuable resources for researchers seeking additional information about the protein's structure, function, genetic associations, and protein-protein interactions.

Research Context and Relevance

1. Post-transcriptional gene regulation mechanisms

2. RNA quality control pathways

3. Stress response regulation through selective mRNA degradation

4. Autophagy regulation pathways

The connection between NUDT20/DCP2 and autophagy regulation suggests potential applications in studying cellular responses to nutrient availability and metabolic stress .