NUDT21 Antibody
Description
Biological Role of NUDT21
NUDT21 (nudix hydrolase 21), also known as CPSF5 or CFIm25, is a subunit of the Cleavage Factor Im (CFIm) complex. It facilitates pre-mRNA 3' cleavage and polyadenylation, a process essential for mRNA stability and nuclear export . NUDT21 localizes to nuclear paraspeckles and interacts with CPSF6/CPSF7 to regulate alternative polyadenylation (APA), influencing transcript diversity and protein expression .
Applications of NUDT21 Antibodies
NUDT21 antibodies are widely used in molecular biology and oncology research. Key applications include:
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Western Blot (WB): Detects NUDT21 at ~26–27 kDa in human, mouse, and rat samples .
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Immunohistochemistry (IHC): Visualizes NUDT21 expression in tissue sections .
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Flow Cytometry (FCM): Analyzes protein levels in leukemic cells .
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Immunoprecipitation (IP): Isolates NUDT21 and its binding partners, such as CPSF6 .
Cancer Biology
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Bladder Cancer (BC): NUDT21 suppresses BC proliferation and metastasis by regulating ANXA2 and LIMK2 3'-UTR lengths, inhibiting Wnt/β-catenin and NF-κB pathways. Overexpression reduces tumor growth in vivo .
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Leukemia: Elevated NUDT21 in chronic myelogenous leukemia (CML) promotes cell proliferation. Knockdown induces apoptosis and G0/G1 cell cycle arrest via ERK1/2 pathway inhibition .
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Pan-Cancer Analysis: High NUDT21 expression correlates with tumor mutational burden (TMB), microsatellite instability (MSI), and immunosuppression through M2 macrophage recruitment .
Cell Fate Regulation
NUDT21 modulates APA of >1,500 transcripts during reprogramming to pluripotency. Its suppression enhances induced pluripotent stem cell (iPSC) generation and disrupts differentiation .
Available NUDT21 Antibody Variants
The table below compares commercially available NUDT21 antibodies:
