NUDT27 Antibody
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Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
NUDT27 antibody; NUDX27 antibody; At5g06340 antibody; MHF15.14 antibody; Nudix hydrolase 27 antibody; chloroplastic antibody; AtNUDT27 antibody; EC 3.6.1.- antibody
Target Names
NUDT27
Uniprot No.
Target Background
Function
NUDT27 Antibody mediates the hydrolysis of certain nucleoside diphosphate derivatives. It can utilize diadenosine 5',5'''-P(1)P(5) pentaphosphate (Ap(5)A) as substrates.
Database Links
Protein Families
Nudix hydrolase family
Subcellular Location
Plastid, chloroplast.
Tissue Specificity
Expressed in roots, leaves, stems and inflorescences.
Q&A
Advanced Research Questions
How to resolve contradictory results between ELISA and functional assays?
Implement quantitative epitope binning:
What computational methods predict cross-reactivity risks for NUDT27 antibodies?
-
Use AlphaFold-Multimer for structural homology modeling against:
Nudix Family Member Sequence Identity NUDT5 38-42% NUDT7 33-37% CD27 (TNFRSF7) <15%
Methodological Recommendations
5. Standardized protocols for quantitative comparison across studies:
How to address batch-to-batch variability in long-term studies?
-
Establish internal reference standards:
Material Storage Stability Testing Lyophilized cell lysate -80°C Quarterly WB validation Recombinant protein aliquots Liquid N2 Annual MS verification
Data Conflict Resolution Framework
7. Systematic approach for reconciling contradictory findings:
| Discrepancy Type | Investigation Protocol |
|---|---|
| Cell-type specific detection | Perform RNA/protein co-expression analysis across 5+ cell lines |
| Subcellular localization mismatch | Conduct fractionation-MS with antibody depletion controls |
| Functional assay divergence | Implement BioLayer interferometry binding-energy calculations |
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