NUDC Human

NudC Nuclear Distribution Protein Human Recombinant
Shipped with Ice Packs
In Stock
Cat. No.
BT1843
Source
Escherichia Coli.
Synonyms
Nuclear Distribution C Dynein Complex Regulator, NudC Nuclear Distribution Protein, Nuclear Distribution Protein C Homolog, Nuclear Distribution Gene C Homolog, Nuclear Distribution C Homolog, Nuclear Migration Protein NudC, NPD011, HNUDC, MNUDC, NUDC.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Quick Inquiry

Description

Research Findings and Disease Implications

Cancer Biology

  • Overexpression: Linked to tumor cell proliferation and migration (e.g., lymphoma, leukemia) .

  • Therapeutic Target: Silencing NUDC disrupts mitosis, suggesting potential in chemotherapy .

Neurodegeneration

  • Retinal Degeneration: NUDC knockout in rod photoreceptors causes rapid functional decline and structural abnormalities .

  • Lissencephaly: Indirect association via LIS1 interaction, though no direct mutations reported .

Chaperone Activity

  • Inhibits protein aggregation independently of Hsp90, akin to sHSPs .

  • Lacks direct binding to LIS1, unlike previous assumptions .

Experimental Models and Clinical Relevance

In Vitro Studies

  • HeLa Cells: NUDCL (a homolog) depletion triggers Golgi disassembly, mitotic defects, and apoptosis .

  • Macrophages: NUDC upregulation correlates with activation states, hinting at immune regulatory roles .

In Vivo Models

  • Mouse Retina: Conditional NUDC knockout in rods leads to:

    • Mislocalized rhodopsin by 3 weeks.

    • Complete functional loss and neurodegeneration by 6 weeks .

Drug Sensitivity

  • NUDCD1 (a paralog) expression correlates with resistance to CTLA-4 inhibitors and chemotherapies in gastric cancer .

Research Tools and Recombinant Forms

Recombinant NUDC (Prospec Bio)

  • Source: E. coli-expressed, His-tagged .

  • Purity: >90% (SDS-PAGE) .

  • Applications: Antibody production, kinase assays, and dynein interaction studies .

Antibodies and Inhibitors

  • Commercial antibodies target C-terminal epitopes for Western blotting .

  • No direct inhibitors exist, but RNAi tools (e.g., pBS/U6-NudCL) validate functional roles .

Unresolved Questions and Future Directions

  • Mechanism in Inflammation: How NUDC modulates cytokine signaling remains unclear .

  • Post-Mitotic Roles: Its function in non-dividing cells (e.g., neurons) needs further exploration .

  • Therapeutic Strategies: Targeting NUDC in cancers without harming normal cells requires precision .

Product Specs

Introduction
NUDC (Nuclear Distribution Gene C Homolog) is a crucial protein involved in cell division and neuronal development. It plays a vital role in mitosis and cytokinesis by regulating spindle formation and microtubule organization. NUDC is essential for proper chromosome segregation during cell division and contributes to cell proliferation. It also plays a role in neurogenesis, the formation of new neurons, and neuronal migration.
Description
This product consists of a recombinant human NUDC protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 354 amino acids (with amino acids 1 to 331 present) and has a molecular weight of 40.6 kDa. The NUDC protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
This NUDC protein solution has a concentration of 1 mg/ml and contains 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For longer storage, freeze the solution at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of this product is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Nuclear Distribution C Dynein Complex Regulator, NudC Nuclear Distribution Protein, Nuclear Distribution Protein C Homolog, Nuclear Distribution Gene C Homolog, Nuclear Distribution C Homolog, Nuclear Migration Protein NudC, NPD011, HNUDC, MNUDC, NUDC.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGGEQEE ERFDGMLLAM AQQHEGGVQE LVNTFFSFLR RKTDFFIGGE EGMAEKLITQ TFSHHNQLAQ KTRREKRARQ EAERREKAER AARLAKEAKS ETSGPQIKEL TDEEAERLQL EIDQKKDAEN HEAQLKNGSL DSPGKQDTEE DEEEDEKDKG KLKPNLGNGA DLPNYRWTQT LSELDLAVPF CVNFRLKGKD MVVDIQRRHL RVGLKGQPAI IDGELYNEVK VEESSWLIED GKVVTVHLEK INKMEWWSRL VSSDPEINTK KINPENSKLS DLDSETRSMV EKMMYDQRQK SMGLPTSDEQ KKQEILKKFM DQHPEMDFSK AKFN.

Product Science Overview

Introduction

NudC (Nuclear Distribution C) is a protein coding gene that plays a crucial role in mitosis and cytokinesis. It was first identified in the filamentous fungus Aspergillus nidulans as an upstream regulator of NudF, whose mammalian homolog is Lissencephaly 1 (Lis1). NudC is conserved from fungi to mammals and is involved in various cellular processes, including nuclear migration, spindle formation during mitosis, and microtubule organization during cytokinesis .

Structure and Expression

The human NudC gene consists of at least 9 exons and 8 introns, spanning approximately 8 kb of the genomic region. The protein is widely expressed in various tissues, including the testis and adrenal glands. It is highly expressed in certain cancer cell lines, such as CNE-2 and HNE-2 cells .

Function and Mechanism

NudC plays an essential role in nuclear migration, which is crucial for the growth, development, and cellular function of eukaryotes. It is a part of the dynein complex and is involved in stabilizing the components of the LIS1/dynein complex. This stabilization is vital for proper cell cycle regulation, cell migration, ciliogenesis, thrombopoiesis, and the inflammatory response .

Recombinant Human NudC Protein

Recombinant human NudC protein is typically expressed in HEK293 cells and is used for various research purposes. The recombinant protein consists of 342 amino acids and has a predicted molecular mass of 39.7 kDa. It is supplied in a sterile buffer and is stable for up to twelve months when stored under appropriate conditions .

Clinical Relevance

NudC and its homologs, NudCL and NudCL2, have been implicated in several clinical conditions. For instance, NudCL is essential for the stability of the dynein intermediate chain and ciliogenesis, while NudCL2 regulates the LIS1/dynein pathway by stabilizing LIS1 with the Hsp90 chaperone. Additionally, a distantly related member of the NudC family, CML66, is a tumor-associated antigen in human leukemia and appears to promote oncogenesis by regulating the IGF-1R-MAPK signaling pathway .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.